Mid-IR biosensor: Detection and fingerprinting of pathogens on gold island functionalized chalcogenide films

Chenxu Yu, Ashtosh Ganjoo, H. Jain, C. G. Pantano, Joseph Irudayaraj

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the surface of IR-transparent Ge-containing chalcogenide glass films were formed via thiol chemistry on 20-nm-thick gold islands. As a first step, the protocol was validated by monitoring fluorescently tagged targets to validate binding. FT-IR spectroscopy confirmed that the coating of the films with 20-nm gold did not have a significant effect on the propagation and penetration of IR evanescent waves through the film. The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR finger-printing Highly selective detection of bacterial targets was achieved at both the species (E. coli vs. S. enteriditis) and strain level (E. coli O157:H7 vs E. coli K12). A mid-infrared approach could thus be used as a biosensor as well as a molecular fingerprinting tool.

Original languageEnglish (US)
Pages (from-to)2500-2506
Number of pages7
JournalAnalytical chemistry
Volume78
Issue number8
DOIs
StatePublished - Apr 15 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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