Mid-IR biosensor

Detection and fingerprinting of pathogens on gold island functionalized chalcogenide films

Chenxu Yu, Ashtosh Ganjoo, H. Jain, Carlo G. Pantano, Joseph Irudayaraj

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the surface of IR-transparent Ge-containing chalcogenide glass films were formed via thiol chemistry on 20-nm-thick gold islands. As a first step, the protocol was validated by monitoring fluorescently tagged targets to validate binding. FT-IR spectroscopy confirmed that the coating of the films with 20-nm gold did not have a significant effect on the propagation and penetration of IR evanescent waves through the film. The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR finger-printing Highly selective detection of bacterial targets was achieved at both the species (E. coli vs. S. enteriditis) and strain level (E. coli O157:H7 vs E. coli K12). A mid-infrared approach could thus be used as a biosensor as well as a molecular fingerprinting tool.

Original languageEnglish (US)
Pages (from-to)2500-2506
Number of pages7
JournalAnalytical Chemistry
Volume78
Issue number8
DOIs
StatePublished - Apr 15 2006

Fingerprint

Pathogens
Biosensors
Gold
Escherichia coli
Salmonella
Antibodies
Sulfhydryl Compounds
Printing
Labels
Infrared spectroscopy
Infrared radiation
Glass
Coatings
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Yu, Chenxu ; Ganjoo, Ashtosh ; Jain, H. ; Pantano, Carlo G. ; Irudayaraj, Joseph. / Mid-IR biosensor : Detection and fingerprinting of pathogens on gold island functionalized chalcogenide films. In: Analytical Chemistry. 2006 ; Vol. 78, No. 8. pp. 2500-2506.
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Mid-IR biosensor : Detection and fingerprinting of pathogens on gold island functionalized chalcogenide films. / Yu, Chenxu; Ganjoo, Ashtosh; Jain, H.; Pantano, Carlo G.; Irudayaraj, Joseph.

In: Analytical Chemistry, Vol. 78, No. 8, 15.04.2006, p. 2500-2506.

Research output: Contribution to journalArticle

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