miFAST: A novel and rapid microRNA target capture method

Ashley M. Poenitzsch Strong, Scott M. Berry, David J. Beebe, Jian Liang Li, Vladimir Spiegelman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.

Original languageEnglish (US)
Pages (from-to)559-566
Number of pages8
JournalMolecular Carcinogenesis
Volume57
Issue number4
DOIs
StatePublished - Apr 2018

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cancer Research

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    Poenitzsch Strong, A. M., Berry, S. M., Beebe, D. J., Li, J. L., & Spiegelman, V. (2018). miFAST: A novel and rapid microRNA target capture method. Molecular Carcinogenesis, 57(4), 559-566. https://doi.org/10.1002/mc.22780