Minimizing human infection from escherichia coli o157:H7 using gumbos

Marsha R. Cole, Min Li, Ravirajsinh Jadeja, Bilal El-Zahab, Daniel Hayes, Jeffery A. Hobden, Marlene E. Janes, Isiah M. Warner

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: Reduction in faecal shedding of Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) in food-producing animals is a viable strategy to minimize human disease initiated by exposure to these microorganisms. To this end, an intervention strategy involving the electrostatic hybridization of two commonly used anti-infective agents for veterinary practice (i.e. chlorhexidine and ampicillin) was evaluated to curtail EHECtransmitted disease from ruminant sources. Chlorhexidine di-ampicillin is a novel group of uniform material based on organic salts (GUMBOS) with inherent in vitro antibacterial activity that comes from its parent antimicrobial ions, chlorhexidine and ampicillin. Methods: Antibacterial activities for chlorhexidine diacetate, sodium ampicillin, chlorhexidine di-ampicillin and stoichiometrically equivalent 1:2 chlorhexidine diacetate:sodium ampicillin were assessed using the serial 2-fold dilution method and time-kill studies against seven isolates of E. coli O157:H7 and one non-pathogenic E. coli 25922. Further studies to investigate synergistic interactions of reacted and stoichiometrically equivalent unreacted antimicrobial agents at MICs and possible mechanisms were also investigated. Results: Synergism and in vitro antibacterial activities against EHEC were observed in this study, which suggests chlorhexidine di-ampicillin could be a useful reagent in reducing EHEC transmission and minimizing EHEC-associated infections. Likewise, chlorhexidine di-ampicillin reduced HeLa cell toxicity as compared with chlorhexidine diacetate or the stoichiometric combination of antimicrobial agents. Further results suggest that the mechanisms of action of chlorhexidine di-ampicillin and chlorhexidine diacetate against E. coli O157:H7 are similar. Conclusions: Reacting antimicrobial GUMBOS as indicated in this study may enhance the approach to current combination drug therapeutic strategies for EHEC disease control and prevention

Original languageEnglish (US)
Pages (from-to)1312-1318
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume68
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Chlorhexidine
Escherichia coli O157
Ampicillin
Enterohemorrhagic Escherichia coli
Infection
Anti-Infective Agents
Salts
Shiga-Toxigenic Escherichia coli
Escherichia coli Infections
Ruminants
Drug Combinations
Static Electricity
HeLa Cells
Ions
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Cole, M. R., Li, M., Jadeja, R., El-Zahab, B., Hayes, D., Hobden, J. A., ... Warner, I. M. (2013). Minimizing human infection from escherichia coli o157:H7 using gumbos. Journal of Antimicrobial Chemotherapy, 68(6), 1312-1318. https://doi.org/10.1093/jac/dkt010
Cole, Marsha R. ; Li, Min ; Jadeja, Ravirajsinh ; El-Zahab, Bilal ; Hayes, Daniel ; Hobden, Jeffery A. ; Janes, Marlene E. ; Warner, Isiah M. / Minimizing human infection from escherichia coli o157:H7 using gumbos. In: Journal of Antimicrobial Chemotherapy. 2013 ; Vol. 68, No. 6. pp. 1312-1318.
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Cole, MR, Li, M, Jadeja, R, El-Zahab, B, Hayes, D, Hobden, JA, Janes, ME & Warner, IM 2013, 'Minimizing human infection from escherichia coli o157:H7 using gumbos', Journal of Antimicrobial Chemotherapy, vol. 68, no. 6, pp. 1312-1318. https://doi.org/10.1093/jac/dkt010

Minimizing human infection from escherichia coli o157:H7 using gumbos. / Cole, Marsha R.; Li, Min; Jadeja, Ravirajsinh; El-Zahab, Bilal; Hayes, Daniel; Hobden, Jeffery A.; Janes, Marlene E.; Warner, Isiah M.

In: Journal of Antimicrobial Chemotherapy, Vol. 68, No. 6, 01.06.2013, p. 1312-1318.

Research output: Contribution to journalArticle

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AU - Cole, Marsha R.

AU - Li, Min

AU - Jadeja, Ravirajsinh

AU - El-Zahab, Bilal

AU - Hayes, Daniel

AU - Hobden, Jeffery A.

AU - Janes, Marlene E.

AU - Warner, Isiah M.

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N2 - Objectives: Reduction in faecal shedding of Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) in food-producing animals is a viable strategy to minimize human disease initiated by exposure to these microorganisms. To this end, an intervention strategy involving the electrostatic hybridization of two commonly used anti-infective agents for veterinary practice (i.e. chlorhexidine and ampicillin) was evaluated to curtail EHECtransmitted disease from ruminant sources. Chlorhexidine di-ampicillin is a novel group of uniform material based on organic salts (GUMBOS) with inherent in vitro antibacterial activity that comes from its parent antimicrobial ions, chlorhexidine and ampicillin. Methods: Antibacterial activities for chlorhexidine diacetate, sodium ampicillin, chlorhexidine di-ampicillin and stoichiometrically equivalent 1:2 chlorhexidine diacetate:sodium ampicillin were assessed using the serial 2-fold dilution method and time-kill studies against seven isolates of E. coli O157:H7 and one non-pathogenic E. coli 25922. Further studies to investigate synergistic interactions of reacted and stoichiometrically equivalent unreacted antimicrobial agents at MICs and possible mechanisms were also investigated. Results: Synergism and in vitro antibacterial activities against EHEC were observed in this study, which suggests chlorhexidine di-ampicillin could be a useful reagent in reducing EHEC transmission and minimizing EHEC-associated infections. Likewise, chlorhexidine di-ampicillin reduced HeLa cell toxicity as compared with chlorhexidine diacetate or the stoichiometric combination of antimicrobial agents. Further results suggest that the mechanisms of action of chlorhexidine di-ampicillin and chlorhexidine diacetate against E. coli O157:H7 are similar. Conclusions: Reacting antimicrobial GUMBOS as indicated in this study may enhance the approach to current combination drug therapeutic strategies for EHEC disease control and prevention

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