MinION nanopore sequencing of an influenza genome

Jing Wang, Nicole E. Moore, Yi Mo Deng, David A. Eccles, Richard J. Hall

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Influenza epidemics and pandemics have significant impacts on economies, morbidity and mortality worldwide. The ability to rapidly and accurately sequence influenza viruses is instrumental in the prevention and mitigation of influenza. All eight influenza genes from an influenza A virus were amplified by PCR simultaneously and then subjected to sequencing on a MinION nanopore sequencer. A complete influenza virus genome was obtained that shared greater than 99% identity with sequence data obtained from Illumina Miseq and traditional Sanger sequencing. The laboratory infrastructure and computing resources used to perform this experiment on the MinION nanopore sequencer would be available in most molecular laboratories around the world. Using this system, the concept of portability, and thus sequencing influenza viruses in the clinic or field is now tenable.

Original languageEnglish (US)
Article number766
JournalFrontiers in Microbiology
Volume6
Issue numberJUL
DOIs
StatePublished - Jan 1 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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