miRNA-mediated auxin signalling repression during Vat-mediated aphid resistance in Cucumis melo

Sampurna Sattar, Charles Addo-Quaye, Gary A. Thompson

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Abstract

Resistance to Aphis gossypii in melon is attributed to the presence of the single dominant R gene virus aphid transmission (Vat), which is biologically expressed as antibiosis, antixenosis and tolerance. However, the mechanism of resistance is poorly understood at the molecular level. Aphid-induced transcriptional changes, including differentially expressed miRNA profiles that correspond to resistance interaction have been reported in melon. The potential regulatory roles of miRNAs in Vat-mediated aphid resistance were further revealed by identifying the specific miRNA degradation targets. A total of 70 miRNA:target pairs, including 28 novel miRNA:target pairs, for the differentially expressed miRNAs were identified: 11 were associated with phytohormone regulation, including six miRNAs that potentially regulate auxin interactions. A model for a redundant regulatory system of miRNA-mediated auxin insensitivity is proposed that incorporates auxin perception, auxin modification and auxin-regulated transcription. Chemically inhibiting the transport inhibitor response-1 (TIR-1) auxin receptor in susceptible melon tissues provides in vivo support for the model of auxin-mediated impacts on A. gossypii resistance.

Original languageEnglish (US)
Pages (from-to)1216-1227
Number of pages12
JournalPlant Cell and Environment
Volume39
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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