Mitochondrial DNA extraction and typing from isolated dentin- experimental evaluation in a Korean population

H. Pfeiffer, R. Steighner, R. Fisher, H. Mörnstad, C. L. Yoon, Mitchell Mark Holland

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

This study reports mtDNA polymorphisms in both hypervariable segments HV1 and HV2 of the non coding D-loop region from 60 unrelated Koreans. In contrast to two previous Korean data base studies, mtDNA was extracted separately from pulp tissue and root dentin of teeth obtained from dentists. Dentin turned out to be a reliable source of mitochondrial DNA. This can be of practical importance in forensic identification case work after a long post-mortem interval since pulp decomposes rapidly. The extraction method is explained in detail. The mtDNA polymorphisms obtained from 60 teeth of unrelated Koreans were compared with the already existing Korean data base.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalInternational Journal of Legal Medicine
Volume111
Issue number6
DOIs
StatePublished - Oct 1 1998

Fingerprint

DNA Fingerprinting
Dentin
Mitochondrial DNA
Population
Databases
Tooth Root
Dentists
Tooth

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Pfeiffer, H. ; Steighner, R. ; Fisher, R. ; Mörnstad, H. ; Yoon, C. L. ; Holland, Mitchell Mark. / Mitochondrial DNA extraction and typing from isolated dentin- experimental evaluation in a Korean population. In: International Journal of Legal Medicine. 1998 ; Vol. 111, No. 6. pp. 309-313.
@article{51cf542fce6c46f7b59d85235857568f,
title = "Mitochondrial DNA extraction and typing from isolated dentin- experimental evaluation in a Korean population",
abstract = "This study reports mtDNA polymorphisms in both hypervariable segments HV1 and HV2 of the non coding D-loop region from 60 unrelated Koreans. In contrast to two previous Korean data base studies, mtDNA was extracted separately from pulp tissue and root dentin of teeth obtained from dentists. Dentin turned out to be a reliable source of mitochondrial DNA. This can be of practical importance in forensic identification case work after a long post-mortem interval since pulp decomposes rapidly. The extraction method is explained in detail. The mtDNA polymorphisms obtained from 60 teeth of unrelated Koreans were compared with the already existing Korean data base.",
author = "H. Pfeiffer and R. Steighner and R. Fisher and H. M{\"o}rnstad and Yoon, {C. L.} and Holland, {Mitchell Mark}",
year = "1998",
month = "10",
day = "1",
doi = "10.1007/s004140050177",
language = "English (US)",
volume = "111",
pages = "309--313",
journal = "International Journal of Legal Medicine",
issn = "0937-9827",
publisher = "Springer Verlag",
number = "6",

}

Mitochondrial DNA extraction and typing from isolated dentin- experimental evaluation in a Korean population. / Pfeiffer, H.; Steighner, R.; Fisher, R.; Mörnstad, H.; Yoon, C. L.; Holland, Mitchell Mark.

In: International Journal of Legal Medicine, Vol. 111, No. 6, 01.10.1998, p. 309-313.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mitochondrial DNA extraction and typing from isolated dentin- experimental evaluation in a Korean population

AU - Pfeiffer, H.

AU - Steighner, R.

AU - Fisher, R.

AU - Mörnstad, H.

AU - Yoon, C. L.

AU - Holland, Mitchell Mark

PY - 1998/10/1

Y1 - 1998/10/1

N2 - This study reports mtDNA polymorphisms in both hypervariable segments HV1 and HV2 of the non coding D-loop region from 60 unrelated Koreans. In contrast to two previous Korean data base studies, mtDNA was extracted separately from pulp tissue and root dentin of teeth obtained from dentists. Dentin turned out to be a reliable source of mitochondrial DNA. This can be of practical importance in forensic identification case work after a long post-mortem interval since pulp decomposes rapidly. The extraction method is explained in detail. The mtDNA polymorphisms obtained from 60 teeth of unrelated Koreans were compared with the already existing Korean data base.

AB - This study reports mtDNA polymorphisms in both hypervariable segments HV1 and HV2 of the non coding D-loop region from 60 unrelated Koreans. In contrast to two previous Korean data base studies, mtDNA was extracted separately from pulp tissue and root dentin of teeth obtained from dentists. Dentin turned out to be a reliable source of mitochondrial DNA. This can be of practical importance in forensic identification case work after a long post-mortem interval since pulp decomposes rapidly. The extraction method is explained in detail. The mtDNA polymorphisms obtained from 60 teeth of unrelated Koreans were compared with the already existing Korean data base.

UR - http://www.scopus.com/inward/record.url?scp=0031752938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031752938&partnerID=8YFLogxK

U2 - 10.1007/s004140050177

DO - 10.1007/s004140050177

M3 - Article

C2 - 9826090

AN - SCOPUS:0031752938

VL - 111

SP - 309

EP - 313

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

SN - 0937-9827

IS - 6

ER -