Mitochondrial DNA from lupine: restriction analysis and cloning of fragments coding for tRNA

Halina Augustyniak, Piotr Borsuk, Iwona Hirschler-Laszkiewicz, Piotr P. Stepień, Ewa Bartnik

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) was isolated from lupine. Restriction analysis was used to estimate its size, which is about 180 kb. A BamHI bank of this mtDNA was constructed using plasmids pBR322 and pBR327 as vectors. Eight clones containing plasmids hybridizing to mitochondrial tRNA (mttRNA) were isolated. Restriction maps of these plasmids were determined. Six of these plasmids hybridized to unique fragments and two to two fragments of very similar size, all obtained by BamHI cleavage of mtDNA.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalGene
Volume22
Issue number1
DOIs
StatePublished - Apr 1983

All Science Journal Classification (ASJC) codes

  • Genetics

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