This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.