Mode of resistance of triazine-resistant annual bluegrass (Poa annua)

Steven T. Kelly, G. Euel Coats, Dawn S. Luthe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Two previously identified triazine-resistant annual bluegrass (Poa annua) biotypes in Mississippi were evaluated to determine the level as well as the mode of resistance compared to a triazine-susceptible biotype. Annual bluegrass seeds were collected at two sites (Meridian and Tupelo, MS) from field plots previously treated with 11.2 kg-ai/ha simazine. Seeds were planted on agar containing simazine (0, 0.001, 0.01, 0.1, 1, 10, or 100 mM) or diuron (0, 0.01, 0.1, 1, 10, 100, or 1,000 μM), placed in the growth chamber with an 8 h daylength and alternating temperatures of 15 and 20 C, and allowed to grow for 3 wk, after which fresh weight was determined. Data were subjected to regression analyses and the amount of simazine or diuron required to reduce fresh weight to 50% of the untreated plants was determined. These data indicated a greater than 1,000-fold level of resistance to simazine with either biotype. Diuron concentrations of 5.0, 3.0, and 3.2 μM were required to reduce fresh weight to 50% of the untreated for the triazine-susceptible, Meridian, and Tupelo biotypes, respectively. Sequencing a portion of the chloroplast psbA gene revealed a serine 264 to glycine mutation, which reduced the affinity of the triazine herbicides for the Q(B)-binding niche on the D1 protein in photosystem II.

Original languageEnglish (US)
Pages (from-to)747-752
Number of pages6
JournalWeed Technology
Volume13
Issue number4
StatePublished - Oct 1 1999

Fingerprint

simazine
Poa annua
triazines
biotypes
diuron
triazine herbicides
D1 protein
seeds
growth chambers
glycine (amino acid)
photosystem II
serine
niches
chloroplasts
photoperiod
agar
mutation
temperature
genes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Kelly, S. T., Coats, G. E., & Luthe, D. S. (1999). Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technology, 13(4), 747-752.
Kelly, Steven T. ; Coats, G. Euel ; Luthe, Dawn S. / Mode of resistance of triazine-resistant annual bluegrass (Poa annua). In: Weed Technology. 1999 ; Vol. 13, No. 4. pp. 747-752.
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Kelly, ST, Coats, GE & Luthe, DS 1999, 'Mode of resistance of triazine-resistant annual bluegrass (Poa annua)', Weed Technology, vol. 13, no. 4, pp. 747-752.

Mode of resistance of triazine-resistant annual bluegrass (Poa annua). / Kelly, Steven T.; Coats, G. Euel; Luthe, Dawn S.

In: Weed Technology, Vol. 13, No. 4, 01.10.1999, p. 747-752.

Research output: Contribution to journalArticle

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N2 - Two previously identified triazine-resistant annual bluegrass (Poa annua) biotypes in Mississippi were evaluated to determine the level as well as the mode of resistance compared to a triazine-susceptible biotype. Annual bluegrass seeds were collected at two sites (Meridian and Tupelo, MS) from field plots previously treated with 11.2 kg-ai/ha simazine. Seeds were planted on agar containing simazine (0, 0.001, 0.01, 0.1, 1, 10, or 100 mM) or diuron (0, 0.01, 0.1, 1, 10, 100, or 1,000 μM), placed in the growth chamber with an 8 h daylength and alternating temperatures of 15 and 20 C, and allowed to grow for 3 wk, after which fresh weight was determined. Data were subjected to regression analyses and the amount of simazine or diuron required to reduce fresh weight to 50% of the untreated plants was determined. These data indicated a greater than 1,000-fold level of resistance to simazine with either biotype. Diuron concentrations of 5.0, 3.0, and 3.2 μM were required to reduce fresh weight to 50% of the untreated for the triazine-susceptible, Meridian, and Tupelo biotypes, respectively. Sequencing a portion of the chloroplast psbA gene revealed a serine 264 to glycine mutation, which reduced the affinity of the triazine herbicides for the Q(B)-binding niche on the D1 protein in photosystem II.

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Kelly ST, Coats GE, Luthe DS. Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technology. 1999 Oct 1;13(4):747-752.