Model multilayer structures for three-dimensional cell imaging

Joseph Kozole, Christopher Szakal, Michael Kurczy, Nicholas Winograd

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The prospects for SIMS three-dimensional analysis of biological materials were explored using model multilayer structures. The samples were analyzed in a ToF-SIMS spectrometer equipped with a 20 keV buckminsterfullerene (C 60 + ) ion source. Molecular depth information was acquired using a C 60 + ion beam to etch through the multilayer structures at specified time intervals. Subsequent to each individual erosion cycle, static SIMS spectra were recorded using a pulsed C 60 + ion probe. Molecular intensities in sequential mass spectra were monitored as a function of primary ion fluence. The resulting depth information was used to characterize C 60 + bombardment of biological materials. Specifically, molecular depth profile studies involving dehydrated dipalmitoyl-phosphatidylcholine (DPPC) organic films indicate that cell membrane lipid materials do not experience significant chemical damage when bombarded with C 60 + ion fluences greater than 10 15 ions/cm 2 . Moreover, depth profile analyses of DPPC-sucrose frozen multilayer structures suggest that biomolecule information can be uncovered after the C 60 + sputter removal of a 20 nm overlayer with no appreciable loss of underlying molecular signal. The experimental results support the potential for three-dimensional molecular mapping of biological materials using cluster SIMS.

Original languageEnglish (US)
Pages (from-to)6789-6792
Number of pages4
JournalApplied Surface Science
Volume252
Issue number19
DOIs
StatePublished - Jul 30 2006

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Secondary ion mass spectrometry
Multilayers
Biological materials
Ions
Imaging techniques
1,2-Dipalmitoylphosphatidylcholine
Fullerenes
Sugar (sucrose)
Biomolecules
Ion sources
Membrane Lipids
Ion beams
Sucrose
Spectrometers
Erosion

All Science Journal Classification (ASJC) codes

  • Surfaces, Coatings and Films

Cite this

Kozole, Joseph ; Szakal, Christopher ; Kurczy, Michael ; Winograd, Nicholas. / Model multilayer structures for three-dimensional cell imaging. In: Applied Surface Science. 2006 ; Vol. 252, No. 19. pp. 6789-6792.
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Model multilayer structures for three-dimensional cell imaging. / Kozole, Joseph; Szakal, Christopher; Kurczy, Michael; Winograd, Nicholas.

In: Applied Surface Science, Vol. 252, No. 19, 30.07.2006, p. 6789-6792.

Research output: Contribution to journalArticle

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