Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa

Zhaobin Xu, Xin Fang, Thomas Keith Wood, Zuyi Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The planktonic-biofilm transition is reported to facilitate the survival of disease-causing pathogens in a hostile environment, as the ability of pathogens to resist antibiotic treatment is 10 to 1000 times higher in a biofilm than in the planktonic mode. The availability of nutrient components such as amino acids in the surrounding environment plays an important role in the initiation of the biofilm formation. This work proposes a metabolic modeling framework to study the influence of availability of amino acids on the biofilm formation capability of Pseudomonas aeruginosa, one of the major pathogens causing nosocomial infections. Specifically, the genes that are up-regulated during the biofilm initiation were used to determine the metabolic reactions that are positively associated with P. aeruginosa biofilm formation. A criterion was then defined from the change of fluxes of these biofilm-associated reactions and the biomass growth rates to quantify the chance of biofilm formation upon the change of amino acid uptake rates. It was found that adding one of the following eleven amino acids, including Arg, Tyr, Phe, His, Iso, Orn, Pro, Glu, Leu, Val, and Asp, into the minimal medium may trigger P. aeruginosa biofilm formation. These results are perfectly consistent with the existing experimental data. The developed modeling framework was further used as an in silico platform to investigate the impact of the availability of two amino acids on the biofilm formation. It was found that the availability of additional amino acids can enhance biofilm formation and that there may be synergistic mechanisms for multiple amino acids to promote the biofilm formation.

Original languageEnglish (US)
Title of host publication19th IFAC World Congress IFAC 2014, Proceedings
EditorsXiaohua Xia, Edward Boje
PublisherIFAC Secretariat
Pages803-808
Number of pages6
ISBN (Electronic)9783902823625
StatePublished - Jan 1 2014
Event19th IFAC World Congress on International Federation of Automatic Control, IFAC 2014 - Cape Town, South Africa
Duration: Aug 24 2014Aug 29 2014

Publication series

NameIFAC Proceedings Volumes (IFAC-PapersOnline)
Volume19
ISSN (Print)1474-6670

Other

Other19th IFAC World Congress on International Federation of Automatic Control, IFAC 2014
CountrySouth Africa
CityCape Town
Period8/24/148/29/14

Fingerprint

Biofilms
Amino acids
Pathogens
Availability
Antibiotics
Nutrients
Biomass
Genes
Fluxes

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Xu, Z., Fang, X., Wood, T. K., & Huang, Z. (2014). Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa. In X. Xia, & E. Boje (Eds.), 19th IFAC World Congress IFAC 2014, Proceedings (pp. 803-808). (IFAC Proceedings Volumes (IFAC-PapersOnline); Vol. 19). IFAC Secretariat.
Xu, Zhaobin ; Fang, Xin ; Wood, Thomas Keith ; Huang, Zuyi. / Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa. 19th IFAC World Congress IFAC 2014, Proceedings. editor / Xiaohua Xia ; Edward Boje. IFAC Secretariat, 2014. pp. 803-808 (IFAC Proceedings Volumes (IFAC-PapersOnline)).
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Xu, Z, Fang, X, Wood, TK & Huang, Z 2014, Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa. in X Xia & E Boje (eds), 19th IFAC World Congress IFAC 2014, Proceedings. IFAC Proceedings Volumes (IFAC-PapersOnline), vol. 19, IFAC Secretariat, pp. 803-808, 19th IFAC World Congress on International Federation of Automatic Control, IFAC 2014, Cape Town, South Africa, 8/24/14.

Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa. / Xu, Zhaobin; Fang, Xin; Wood, Thomas Keith; Huang, Zuyi.

19th IFAC World Congress IFAC 2014, Proceedings. ed. / Xiaohua Xia; Edward Boje. IFAC Secretariat, 2014. p. 803-808 (IFAC Proceedings Volumes (IFAC-PapersOnline); Vol. 19).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Xu Z, Fang X, Wood TK, Huang Z. Modeling framework for investigating the influence of amino acids on the planktonic-biofilm transition of Pseudomonas aeruginosa. In Xia X, Boje E, editors, 19th IFAC World Congress IFAC 2014, Proceedings. IFAC Secretariat. 2014. p. 803-808. (IFAC Proceedings Volumes (IFAC-PapersOnline)).