Modeling of nisin production by Lactococcus lactis

Thunyarat Pongtharangkul, Ali Demirci, Virendra Puri

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation showed that they could be used for the prediction accurately (slope = 1.01, R-square = 0.99). For nisin production, the Luedeking-Piret model fit considerably well and could be successfully use to predict nisin production from the value of biomass (slope = 1.04, R-square = 0.98).

Original languageEnglish (US)
Title of host publication2007 ASABE Annual International Meeting, Technical Papers
Volume14 BOOK
StatePublished - 2007
Event2007 ASABE Annual International Meeting, Technical Papers - Minneapolis, MN, United States
Duration: Jun 17 2007Jun 20 2007

Other

Other2007 ASABE Annual International Meeting, Technical Papers
CountryUnited States
CityMinneapolis, MN
Period6/17/076/20/07

Fingerprint

Nisin
Lactococcus lactis
nisin
Fermentation
Biomass
Lactic acid
Batch cell culture
Bacteria
Food preservatives
fermentation
Enzyme kinetics
Sugar (sucrose)
Lactic Acid
biomass
Food Preservatives
Logistics
food preservatives
Lactococcus lactis subsp. lactis
Bacteriocins
Batch Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Engineering(all)

Cite this

Pongtharangkul, T., Demirci, A., & Puri, V. (2007). Modeling of nisin production by Lactococcus lactis. In 2007 ASABE Annual International Meeting, Technical Papers (Vol. 14 BOOK)
Pongtharangkul, Thunyarat ; Demirci, Ali ; Puri, Virendra. / Modeling of nisin production by Lactococcus lactis. 2007 ASABE Annual International Meeting, Technical Papers. Vol. 14 BOOK 2007.
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Pongtharangkul, T, Demirci, A & Puri, V 2007, Modeling of nisin production by Lactococcus lactis. in 2007 ASABE Annual International Meeting, Technical Papers. vol. 14 BOOK, 2007 ASABE Annual International Meeting, Technical Papers, Minneapolis, MN, United States, 6/17/07.

Modeling of nisin production by Lactococcus lactis. / Pongtharangkul, Thunyarat; Demirci, Ali; Puri, Virendra.

2007 ASABE Annual International Meeting, Technical Papers. Vol. 14 BOOK 2007.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Modeling of nisin production by Lactococcus lactis

AU - Pongtharangkul, Thunyarat

AU - Demirci, Ali

AU - Puri, Virendra

PY - 2007

Y1 - 2007

N2 - Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation showed that they could be used for the prediction accurately (slope = 1.01, R-square = 0.99). For nisin production, the Luedeking-Piret model fit considerably well and could be successfully use to predict nisin production from the value of biomass (slope = 1.04, R-square = 0.98).

AB - Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation showed that they could be used for the prediction accurately (slope = 1.01, R-square = 0.99). For nisin production, the Luedeking-Piret model fit considerably well and could be successfully use to predict nisin production from the value of biomass (slope = 1.04, R-square = 0.98).

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M3 - Conference contribution

VL - 14 BOOK

BT - 2007 ASABE Annual International Meeting, Technical Papers

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Pongtharangkul T, Demirci A, Puri V. Modeling of nisin production by Lactococcus lactis. In 2007 ASABE Annual International Meeting, Technical Papers. Vol. 14 BOOK. 2007