Modeling of vitamin K (Menaquinoe-7) fermentation by Bacillus subtilis natto in biofilm reactors

Ehsan Mahdinia, Sina Jahangiri Mamouri, Virendra Puri, Ali Demirci, Aydin Berenjian

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Menaquinoe-7 (MK-7) is the most potent form of vitamin K with numerous benefits for human health such as reducing the risks of cardiovascular diseases and osteoporosis in addition to having antitumor characteristics. Therefore, MK-7 production via bacterial fermentation has been studied intensely in the last few decades. Recently, biofilm reactors were implemented to enhance production levels in our lab. The specific objective of this study was to mathematically model the findings in batch biofilm reactors and thus further deepen our understanding of the conditions governing fermentation to pave the path to scaling up the production to pilot scale fermenters. The modified logistic equation was used to correlate substrate consumption with fermentation time and to model the substrate consumption in the four batch fermentations. Results indicated that this modified logistic model fits well with the experimental data for substrate consumption. Therefore there was no need for the other more complex models. Furthermore, this successfully modified-logistic equation was inserted into the Luedeking-Piret model to estimate MK-7 production based on substrate consumption, because of the typical MK-7 profiles in batch fermentations. There is sufficient evidence in the literature that MK-7 biosynthesis follows a mixed-metabolite pattern in Bacillus subtilis natto. Similarly, the modified-Gompertz model was used also for the same purpose. Results indicated that the modified Luedeking-Piret model fits more accurately for MK-7 production (R2 = 0.971, 0.943, 0.970, and 0.959) compared to the modified-Gompertz (R2 = 0.914, 0.943, 0.949 and 0.860).

Original languageEnglish (US)
Pages (from-to)196-202
Number of pages7
JournalBiocatalysis and Agricultural Biotechnology
Volume17
DOIs
StatePublished - Jan 1 2019

Fingerprint

natto
vitamin K
Vitamin K
Vitamins
Biofilms
Bacilli
Bacillus subtilis
biofilm
Fermentation
fermentation
batch fermentation
Logistics
Substrates
Insurance Benefits
Fermenters
fermenters
osteoporosis
Osteoporosis
logit analysis
Biosynthesis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Modeling of vitamin K (Menaquinoe-7) fermentation by Bacillus subtilis natto in biofilm reactors",
abstract = "Menaquinoe-7 (MK-7) is the most potent form of vitamin K with numerous benefits for human health such as reducing the risks of cardiovascular diseases and osteoporosis in addition to having antitumor characteristics. Therefore, MK-7 production via bacterial fermentation has been studied intensely in the last few decades. Recently, biofilm reactors were implemented to enhance production levels in our lab. The specific objective of this study was to mathematically model the findings in batch biofilm reactors and thus further deepen our understanding of the conditions governing fermentation to pave the path to scaling up the production to pilot scale fermenters. The modified logistic equation was used to correlate substrate consumption with fermentation time and to model the substrate consumption in the four batch fermentations. Results indicated that this modified logistic model fits well with the experimental data for substrate consumption. Therefore there was no need for the other more complex models. Furthermore, this successfully modified-logistic equation was inserted into the Luedeking-Piret model to estimate MK-7 production based on substrate consumption, because of the typical MK-7 profiles in batch fermentations. There is sufficient evidence in the literature that MK-7 biosynthesis follows a mixed-metabolite pattern in Bacillus subtilis natto. Similarly, the modified-Gompertz model was used also for the same purpose. Results indicated that the modified Luedeking-Piret model fits more accurately for MK-7 production (R2 = 0.971, 0.943, 0.970, and 0.959) compared to the modified-Gompertz (R2 = 0.914, 0.943, 0.949 and 0.860).",
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Modeling of vitamin K (Menaquinoe-7) fermentation by Bacillus subtilis natto in biofilm reactors. / Mahdinia, Ehsan; Mamouri, Sina Jahangiri; Puri, Virendra; Demirci, Ali; Berenjian, Aydin.

In: Biocatalysis and Agricultural Biotechnology, Vol. 17, 01.01.2019, p. 196-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modeling of vitamin K (Menaquinoe-7) fermentation by Bacillus subtilis natto in biofilm reactors

AU - Mahdinia, Ehsan

AU - Mamouri, Sina Jahangiri

AU - Puri, Virendra

AU - Demirci, Ali

AU - Berenjian, Aydin

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Y1 - 2019/1/1

N2 - Menaquinoe-7 (MK-7) is the most potent form of vitamin K with numerous benefits for human health such as reducing the risks of cardiovascular diseases and osteoporosis in addition to having antitumor characteristics. Therefore, MK-7 production via bacterial fermentation has been studied intensely in the last few decades. Recently, biofilm reactors were implemented to enhance production levels in our lab. The specific objective of this study was to mathematically model the findings in batch biofilm reactors and thus further deepen our understanding of the conditions governing fermentation to pave the path to scaling up the production to pilot scale fermenters. The modified logistic equation was used to correlate substrate consumption with fermentation time and to model the substrate consumption in the four batch fermentations. Results indicated that this modified logistic model fits well with the experimental data for substrate consumption. Therefore there was no need for the other more complex models. Furthermore, this successfully modified-logistic equation was inserted into the Luedeking-Piret model to estimate MK-7 production based on substrate consumption, because of the typical MK-7 profiles in batch fermentations. There is sufficient evidence in the literature that MK-7 biosynthesis follows a mixed-metabolite pattern in Bacillus subtilis natto. Similarly, the modified-Gompertz model was used also for the same purpose. Results indicated that the modified Luedeking-Piret model fits more accurately for MK-7 production (R2 = 0.971, 0.943, 0.970, and 0.959) compared to the modified-Gompertz (R2 = 0.914, 0.943, 0.949 and 0.860).

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