Modification of luciferase to be a substrate for plant aspartic proteinase

Wendy J. Amidon, Joanne E. Pfeil, Susannah Gal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The possibility of using firefly luciferase as a substrate for an aspartic proteinase was explored. Several amino acid modifications to the C-terminus of the luciferase were created on the basis of the known substrate of the Arabidopsis thaliana aspartic proteinase, pro-(barley lectin). One luciferase with the sequence Arg-Asp-Gly-Val-Phe-Ala-Ala instead of the native Arg-Glu-Ile-Leu-Ile-Lys-Ala at position -15 to -9 relative to the C-terminus of native luciferase was found to possess 17% of the original luciferase activity. When this modified luciferase was incubated with the aspartic proteinase, a specific loss in activity occurred that was not observed with the original luciferase. However, both enzymes seemed very sensitive to the acidic conditions required for aspartic proteinase activity. The other versions of luciferase with different numbers of pro-(barley lectin) amino acids were not active luciferases. This provided information on the structural requirements of the C-terminal portion of the protein for luciferase activity. The luciferase proteins were also monitored during the digestion by using Western blots and some were shown to be substrates for the aspartic proteinase. Contrary to what had been expected, the modified luciferase that incorporated the pro-(barley lectin) sequences was not simply cleaved at the engineered site but at additional positions in the protein. The Arabidopsis aspartic proteinase cleaved two other standard protein substrates at many sites, suggesting that this proteinase could have a role in the degradation of proteins in addition to processing propeptides in plants.

Original languageEnglish (US)
Pages (from-to)425-433
Number of pages9
JournalBiochemical Journal
Volume343
Issue number2
DOIs
StatePublished - Oct 15 1999

Fingerprint

Aspartic Acid Proteases
Luciferases
Substrates
Proteins
Arabidopsis
Firefly Luciferases
Amino Acids
Proteolysis
Digestion
Peptide Hydrolases
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Amidon, Wendy J. ; Pfeil, Joanne E. ; Gal, Susannah. / Modification of luciferase to be a substrate for plant aspartic proteinase. In: Biochemical Journal. 1999 ; Vol. 343, No. 2. pp. 425-433.
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Modification of luciferase to be a substrate for plant aspartic proteinase. / Amidon, Wendy J.; Pfeil, Joanne E.; Gal, Susannah.

In: Biochemical Journal, Vol. 343, No. 2, 15.10.1999, p. 425-433.

Research output: Contribution to journalArticle

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