TY - JOUR
T1 - Modified ligands to F(A) and F(B) in photosystem I. II. Characterization of a mixed ligand [4Fe-4S] cluster in the C51D mutant of PsaC upon rebinding to P700-F(X) cores
AU - Yu, L.
AU - Vassiliev, I. R.
AU - Jung, Y. S.
AU - Bryant, D. A.
AU - Golbeck, J. H.
PY - 1995
Y1 - 1995
N2 - A Photosystem I (PS I) complex reconstituted with PsaC-C51D (aspartate in lieu of cysteine in position 51) shows light-induced EPR signals with g values, line widths, and photoreduction behavior characteristic of F(B). Contrary to an earlier report, a [3Fe-4S] cluster was not located in the reconstituted PS I complex. Instead, a second set of resonances with g values of 2.044, 1.942, and 1.853 becomes EPR-visible when the C51D-PS I complex is measured at 4.2 K. This fast relaxing center, termed F(A)' is likely to represent a [4Fe-4S] cluster in the mixed ligand (3Cys-1Asp) site. Redox studies show that the E(m) of F(A)' and F(B) are -630 mV and -575 mV, respectively. Room temperature optical studies support the presence of two functioning electron acceptors subsequent to F(X), and NADP+ photoreduction rates mediated by ferredoxin and flavodoxin are nearly equivalent to the wild type. In addition to [3Fe-4S] clusters and S = 1/2 ground state [4Fe-4S] clusters, the free PsaC-C51D protein shows resonances near g = 5.5, which may represent a population of high spin (S = 3/2) [4Fe-4S] clusters in the mixed ligand F(A)' site. Similar to the C14D-PS I mutant complex, it is proposed that the P700-F(X) core selectively rebinds those free PsaC-C51D proteins that contain two [4Fe-4S] clusters. These studies show that primary photochemistry and electron transfer rates in PS I are relatively unaffected by the presence of a highly reducing, mixed ligand cluster in the F(A)' site.
AB - A Photosystem I (PS I) complex reconstituted with PsaC-C51D (aspartate in lieu of cysteine in position 51) shows light-induced EPR signals with g values, line widths, and photoreduction behavior characteristic of F(B). Contrary to an earlier report, a [3Fe-4S] cluster was not located in the reconstituted PS I complex. Instead, a second set of resonances with g values of 2.044, 1.942, and 1.853 becomes EPR-visible when the C51D-PS I complex is measured at 4.2 K. This fast relaxing center, termed F(A)' is likely to represent a [4Fe-4S] cluster in the mixed ligand (3Cys-1Asp) site. Redox studies show that the E(m) of F(A)' and F(B) are -630 mV and -575 mV, respectively. Room temperature optical studies support the presence of two functioning electron acceptors subsequent to F(X), and NADP+ photoreduction rates mediated by ferredoxin and flavodoxin are nearly equivalent to the wild type. In addition to [3Fe-4S] clusters and S = 1/2 ground state [4Fe-4S] clusters, the free PsaC-C51D protein shows resonances near g = 5.5, which may represent a population of high spin (S = 3/2) [4Fe-4S] clusters in the mixed ligand F(A)' site. Similar to the C14D-PS I mutant complex, it is proposed that the P700-F(X) core selectively rebinds those free PsaC-C51D proteins that contain two [4Fe-4S] clusters. These studies show that primary photochemistry and electron transfer rates in PS I are relatively unaffected by the presence of a highly reducing, mixed ligand cluster in the F(A)' site.
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U2 - 10.1074/jbc.270.47.28118
DO - 10.1074/jbc.270.47.28118
M3 - Article
C2 - 7499300
AN - SCOPUS:0028972294
VL - 270
SP - 28118
EP - 28125
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 47
ER -