Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase

Jiaxu Li, Toshinori Kinoshita, Sona Pandey, Carl K.Y. Ng, Steven P. Gygi, Ken Ichiro Shimazaki, Sarah Mary Assmann

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Protein kinases are involved in stress signalling in both plant and animal systems. The hormone abscisic acid mediates the responses of plants to stresses such as drought, salinity and cold. Abscisic-acid-activated protein kinase (AAPK)-found in guard cells, which control stomatal pores-has been shown to regulate plasma membrane ion channels. Here we show that AAPK-interacting protein 1 (AKIP1), with sequence homology to heterogeneous nuclear RNA-binding protein A/B, is a substrate of AAPK. AAPK-dependent phosphorylation is required for the interaction of AKIP1 with messenger RNA that encodes dehydrin, a protein implicated in cell protection under stress conditions. AAPK and AKIP1 are present in the guard-cell nucleus, and in vivo treatment of such cells with abscisic acid enhances the partitioning of AKIP1 into subnuclear foci which are reminiscent of nuclear speckles. These results show that phosphorylation-regulated RNA target discrimination by heterogeneous nuclear RNA-binding proteins may be a general phenomenon in eukaryotes, and implicate a plant hormone in the regulation of protein dynamics during rapid subnuclear reorganization.

Original languageEnglish (US)
Pages (from-to)793-797
Number of pages5
JournalNature
Volume418
Issue number6899
DOIs
StatePublished - Aug 15 2002

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Abscisic Acid
RNA-Binding Proteins
Protein Kinases
Heterogeneous Nuclear RNA
Proteins
Acids
Nuclear Proteins
Ion Channels
Phosphorylation
Plant Growth Regulators
Cytoprotection
Droughts
Salinity
Staphylococcal Protein A
Sequence Homology
Eukaryota
Cell Nucleus
Cell Membrane
Hormones
RNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Li, J., Kinoshita, T., Pandey, S., Ng, C. K. Y., Gygi, S. P., Shimazaki, K. I., & Assmann, S. M. (2002). Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature, 418(6899), 793-797. https://doi.org/10.1038/nature00936
Li, Jiaxu ; Kinoshita, Toshinori ; Pandey, Sona ; Ng, Carl K.Y. ; Gygi, Steven P. ; Shimazaki, Ken Ichiro ; Assmann, Sarah Mary. / Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. In: Nature. 2002 ; Vol. 418, No. 6899. pp. 793-797.
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Li, J, Kinoshita, T, Pandey, S, Ng, CKY, Gygi, SP, Shimazaki, KI & Assmann, SM 2002, 'Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase', Nature, vol. 418, no. 6899, pp. 793-797. https://doi.org/10.1038/nature00936

Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. / Li, Jiaxu; Kinoshita, Toshinori; Pandey, Sona; Ng, Carl K.Y.; Gygi, Steven P.; Shimazaki, Ken Ichiro; Assmann, Sarah Mary.

In: Nature, Vol. 418, No. 6899, 15.08.2002, p. 793-797.

Research output: Contribution to journalArticle

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Li J, Kinoshita T, Pandey S, Ng CKY, Gygi SP, Shimazaki KI et al. Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature. 2002 Aug 15;418(6899):793-797. https://doi.org/10.1038/nature00936