Modulation of glucose metabolic response to endotoxin by granulocyte colony-stimulating factor

C. H. Lang, G. J. Bagby, C. Dobrescu, S. Nelson, J. J. Spitzer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present study examines whether in vivo administration of granulocyte colony-stimulating factor (G-CSF) and the resultant neutrophilia alters basal glucose metabolism or modulates the glucose metabolic response to a subsequent endotoxin [lipopolysaccharide (LPS)] challenge. Rats were injected with human recombinant G-CSF (50 μg/kg sc) twice daily for 2 days preceding an injection of LPS. Animals treated with G-CSF showed an eightfold increase in blood polymorphonuclear leukocytes (PMNs) but no detectable changes in hemodynamics or glucose metabolism. In control animals, LPS transiently decreased circulating PMN number, but by 4 h neutrophils had returned to control levels. LPS produced a greater reduction in circulating neutrophils in G-CSF-treated animals, which did not return to pretreatment levels by 4 h. G-CSF also produced marked changes in the glucose metabolic response to LPS. Rates of whole body glucose production and utilization in both control and G- CSF-treated rats were rapidly increased by LPS; however, the increment in glucose flux was 55-100% greater in the latter group. The enhanced rate of hepatic glucose production in this group occurred despite lower plasma levels of lactate and glucagon. The elevated rate of whole body glucose utilization was attributable to the G-CSF-enhanced LPS-induced increase in glucose uptake by the ileum, spleen, liver, and lung. Furthermore, LPS increased glucose uptake by skeletal muscle in G-CSF-treated rats but not in control animals. The enhanced glucose disposal in G-CSF-treated rats was not mediated by increases in plasma glucose or insulin concentrations. Whereas the elevated glucose uptake by the liver and lung in rats given G-CSF was associated with an increased number of tissue neutrophils, as assessed by myeloperoxidase activity, sequestration of neutrophils could not account for the changes in glucose uptake by skeletal muscle. These data suggest that the chronic in vivo administration of G-CSF alone does not alter cellular glucose metabolism but does ready cells so that the typical LPS-induced increase in glucose uptake is amplified. Thus G-CSF may be an important biological response modifier under in vivo conditions.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume263
Issue number5 32-5
StatePublished - Jan 1 1992

Fingerprint

Granulocyte Colony-Stimulating Factor
Endotoxins
Glucose
Lipopolysaccharides
Neutrophils
Liver
Skeletal Muscle
Basal Metabolism
Lung
Immunologic Factors
Glucagon
Ileum
Peroxidase

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

@article{d2646a54a2f14813a743b1b262efc4cd,
title = "Modulation of glucose metabolic response to endotoxin by granulocyte colony-stimulating factor",
abstract = "The present study examines whether in vivo administration of granulocyte colony-stimulating factor (G-CSF) and the resultant neutrophilia alters basal glucose metabolism or modulates the glucose metabolic response to a subsequent endotoxin [lipopolysaccharide (LPS)] challenge. Rats were injected with human recombinant G-CSF (50 μg/kg sc) twice daily for 2 days preceding an injection of LPS. Animals treated with G-CSF showed an eightfold increase in blood polymorphonuclear leukocytes (PMNs) but no detectable changes in hemodynamics or glucose metabolism. In control animals, LPS transiently decreased circulating PMN number, but by 4 h neutrophils had returned to control levels. LPS produced a greater reduction in circulating neutrophils in G-CSF-treated animals, which did not return to pretreatment levels by 4 h. G-CSF also produced marked changes in the glucose metabolic response to LPS. Rates of whole body glucose production and utilization in both control and G- CSF-treated rats were rapidly increased by LPS; however, the increment in glucose flux was 55-100{\%} greater in the latter group. The enhanced rate of hepatic glucose production in this group occurred despite lower plasma levels of lactate and glucagon. The elevated rate of whole body glucose utilization was attributable to the G-CSF-enhanced LPS-induced increase in glucose uptake by the ileum, spleen, liver, and lung. Furthermore, LPS increased glucose uptake by skeletal muscle in G-CSF-treated rats but not in control animals. The enhanced glucose disposal in G-CSF-treated rats was not mediated by increases in plasma glucose or insulin concentrations. Whereas the elevated glucose uptake by the liver and lung in rats given G-CSF was associated with an increased number of tissue neutrophils, as assessed by myeloperoxidase activity, sequestration of neutrophils could not account for the changes in glucose uptake by skeletal muscle. These data suggest that the chronic in vivo administration of G-CSF alone does not alter cellular glucose metabolism but does ready cells so that the typical LPS-induced increase in glucose uptake is amplified. Thus G-CSF may be an important biological response modifier under in vivo conditions.",
author = "Lang, {C. H.} and Bagby, {G. J.} and C. Dobrescu and S. Nelson and Spitzer, {J. J.}",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "263",
journal = "American Journal of Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "5 32-5",

}

Modulation of glucose metabolic response to endotoxin by granulocyte colony-stimulating factor. / Lang, C. H.; Bagby, G. J.; Dobrescu, C.; Nelson, S.; Spitzer, J. J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 263, No. 5 32-5, 01.01.1992.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modulation of glucose metabolic response to endotoxin by granulocyte colony-stimulating factor

AU - Lang, C. H.

AU - Bagby, G. J.

AU - Dobrescu, C.

AU - Nelson, S.

AU - Spitzer, J. J.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The present study examines whether in vivo administration of granulocyte colony-stimulating factor (G-CSF) and the resultant neutrophilia alters basal glucose metabolism or modulates the glucose metabolic response to a subsequent endotoxin [lipopolysaccharide (LPS)] challenge. Rats were injected with human recombinant G-CSF (50 μg/kg sc) twice daily for 2 days preceding an injection of LPS. Animals treated with G-CSF showed an eightfold increase in blood polymorphonuclear leukocytes (PMNs) but no detectable changes in hemodynamics or glucose metabolism. In control animals, LPS transiently decreased circulating PMN number, but by 4 h neutrophils had returned to control levels. LPS produced a greater reduction in circulating neutrophils in G-CSF-treated animals, which did not return to pretreatment levels by 4 h. G-CSF also produced marked changes in the glucose metabolic response to LPS. Rates of whole body glucose production and utilization in both control and G- CSF-treated rats were rapidly increased by LPS; however, the increment in glucose flux was 55-100% greater in the latter group. The enhanced rate of hepatic glucose production in this group occurred despite lower plasma levels of lactate and glucagon. The elevated rate of whole body glucose utilization was attributable to the G-CSF-enhanced LPS-induced increase in glucose uptake by the ileum, spleen, liver, and lung. Furthermore, LPS increased glucose uptake by skeletal muscle in G-CSF-treated rats but not in control animals. The enhanced glucose disposal in G-CSF-treated rats was not mediated by increases in plasma glucose or insulin concentrations. Whereas the elevated glucose uptake by the liver and lung in rats given G-CSF was associated with an increased number of tissue neutrophils, as assessed by myeloperoxidase activity, sequestration of neutrophils could not account for the changes in glucose uptake by skeletal muscle. These data suggest that the chronic in vivo administration of G-CSF alone does not alter cellular glucose metabolism but does ready cells so that the typical LPS-induced increase in glucose uptake is amplified. Thus G-CSF may be an important biological response modifier under in vivo conditions.

AB - The present study examines whether in vivo administration of granulocyte colony-stimulating factor (G-CSF) and the resultant neutrophilia alters basal glucose metabolism or modulates the glucose metabolic response to a subsequent endotoxin [lipopolysaccharide (LPS)] challenge. Rats were injected with human recombinant G-CSF (50 μg/kg sc) twice daily for 2 days preceding an injection of LPS. Animals treated with G-CSF showed an eightfold increase in blood polymorphonuclear leukocytes (PMNs) but no detectable changes in hemodynamics or glucose metabolism. In control animals, LPS transiently decreased circulating PMN number, but by 4 h neutrophils had returned to control levels. LPS produced a greater reduction in circulating neutrophils in G-CSF-treated animals, which did not return to pretreatment levels by 4 h. G-CSF also produced marked changes in the glucose metabolic response to LPS. Rates of whole body glucose production and utilization in both control and G- CSF-treated rats were rapidly increased by LPS; however, the increment in glucose flux was 55-100% greater in the latter group. The enhanced rate of hepatic glucose production in this group occurred despite lower plasma levels of lactate and glucagon. The elevated rate of whole body glucose utilization was attributable to the G-CSF-enhanced LPS-induced increase in glucose uptake by the ileum, spleen, liver, and lung. Furthermore, LPS increased glucose uptake by skeletal muscle in G-CSF-treated rats but not in control animals. The enhanced glucose disposal in G-CSF-treated rats was not mediated by increases in plasma glucose or insulin concentrations. Whereas the elevated glucose uptake by the liver and lung in rats given G-CSF was associated with an increased number of tissue neutrophils, as assessed by myeloperoxidase activity, sequestration of neutrophils could not account for the changes in glucose uptake by skeletal muscle. These data suggest that the chronic in vivo administration of G-CSF alone does not alter cellular glucose metabolism but does ready cells so that the typical LPS-induced increase in glucose uptake is amplified. Thus G-CSF may be an important biological response modifier under in vivo conditions.

UR - http://www.scopus.com/inward/record.url?scp=0026475595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026475595&partnerID=8YFLogxK

M3 - Article

C2 - 1279993

AN - SCOPUS:0026475595

VL - 263

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6119

IS - 5 32-5

ER -