Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice

Xia Xiao, Beng San Yeoh, Piu Saha, Yuan Tian, Vishal Singh, Andrew David Patterson, Matam Vijay Kumar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mammalian siderophores are believed to play a critical role in maintaining iron homeostasis. However, the properties and functions of mammalian siderophores have not been fully clarified. In this study, we have employed Chrome Azurol S (CAS) assay which is a well-established method for bacterial siderophores study, to detect and quantify mammalian siderophores in urine samples. Our study demonstrates that siderophores in urine can be altered by diet, gut microbiota and inflammation. C57BL/6 mice, fed on plant-based chow diets which contain numerous phytochemicals, have more siderophores in the urine compared to those fed on purified diets. Urinary siderophores were up-regulated in iron overload conditions, but not altered by other tested nutrients status. Further, germ-free mice displayed 50% reduced urinary siderophores, in comparison to conventional mice, indicating microbiota biotransformation is critical in generating or stimulating host metabolism to create more siderophores. Altered urinary siderophores levels during inflammation suggest that host health conditions influence systemic siderophores level. This is the first report to measure urinary siderophores as a whole, describing how siderophores levels are modulated under different physiological conditions. We believe that our study opens up a new field in mammalian siderophores research and the technique we used in a novel manner has the potential to be applied to clinical purpose.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalJournal of Nutritional Biochemistry
Volume41
DOIs
StatePublished - Mar 1 2017

Fingerprint

Siderophores
Nutrition
Modulation
Diet
Inflammation
Urine
Gastrointestinal Microbiome
Iron
Iron Overload
Microbiota
Phytochemicals
Biotransformation
Inbred C57BL Mouse
Metabolism
Nutrients

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Xiao, Xia ; Yeoh, Beng San ; Saha, Piu ; Tian, Yuan ; Singh, Vishal ; Patterson, Andrew David ; Kumar, Matam Vijay. / Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice. In: Journal of Nutritional Biochemistry. 2017 ; Vol. 41. pp. 25-33.
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Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice. / Xiao, Xia; Yeoh, Beng San; Saha, Piu; Tian, Yuan; Singh, Vishal; Patterson, Andrew David; Kumar, Matam Vijay.

In: Journal of Nutritional Biochemistry, Vol. 41, 01.03.2017, p. 25-33.

Research output: Contribution to journalArticle

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