Modulations in gene expression and mapping of genes associated with cyst nematode infection of soybean

Z. Vaghchhipawala, R. Bassüner, K. Clayton, K. Lewers, R. Shoemaker, Sally Mackenzie

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Infection of the soybean root by the soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) induces a well-documented, yet poorly understood, response by the host plant. The plant response, involving the differentiation of a feeding structure, or "syncytium," facilitates the feeding and reproduction of the nematode to the detriment of the host. We used a genetic system involving a single dominant soybean gene conferring susceptibility to an inbred nematode strain, VL1, te characterize the nematode-host interaction in susceptible line PI 89008. The restriction fragment length polymorphism marker pB053, shown to map to a major SCN resistance locus, cesegregates with resistance among F2 progeny from the PI 89008 × PI 88287 cross. Cytological examination of the infection process confirmed that syncytium development in this genetic system is similar to that reported by others who used noninbred nematode lines. Our study of infected root tissue in the susceptible line PI 89008 revealed a number of genes enhanced in expression. Among these are catalase, cyclin, elongation factor 1α, β-1,3-endoglucanase, hydroxymethylglutaryl coenzyme A reductase, heat shock protein 70, late embryonic abundant protein 14, and formylglycinamidine ribonucleotide synthase, all of which we have genetically positioned on the public linkage map of soybean. Formylglycinamidine ribonucleotide synthase was found to be tightly linked with a major quantitative trait locus for SCN resistance. Our observations are consistent with the hypothesis proposed by others that feeding site development involves the dramatic modulation of gene expression relative to surrounding root cells.

Original languageEnglish (US)
Pages (from-to)42-54
Number of pages13
JournalMolecular Plant-Microbe Interactions
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2001

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Nematode Infections
Heterodera glycines
cyst nematodes
nematode infections
Chromosome Mapping
Soybeans
chromosome mapping
Cysts
Nematoda
ribonucleotides
soybeans
Gene Expression
gene expression
giant cells
Genes
Ribonucleotides
genes
Giant Cells
cyclins
endo-1,4-beta-glucanase

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Vaghchhipawala, Z. ; Bassüner, R. ; Clayton, K. ; Lewers, K. ; Shoemaker, R. ; Mackenzie, Sally. / Modulations in gene expression and mapping of genes associated with cyst nematode infection of soybean. In: Molecular Plant-Microbe Interactions. 2001 ; Vol. 14, No. 1. pp. 42-54.
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Modulations in gene expression and mapping of genes associated with cyst nematode infection of soybean. / Vaghchhipawala, Z.; Bassüner, R.; Clayton, K.; Lewers, K.; Shoemaker, R.; Mackenzie, Sally.

In: Molecular Plant-Microbe Interactions, Vol. 14, No. 1, 01.01.2001, p. 42-54.

Research output: Contribution to journalArticle

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