Molecular analysis of the sex chromosomes of the platyfish Xiphophorus maculatus: Towards the identification of a new type of master sexual regulator in vertebrates.

Astrid Böhne, Christina Schultheis, Delphine Galiana-Arnoux, Alexander Froschauer, Qingchun Zhou, Cornelia Schmidt, Yvonne Selz, Catherine Ozouf-Costaz, Agnès Dettai, Béatrice Segurens, Arnaud Couloux, Sylvie Bernard-Samain, Valérie Barbe, Stefan Chilmonczyk, Frédéric Brunet, Amandine Darras, Marta Hoover, Marie Semon, Manfred Schartl, Jean Nicolas Volff

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In contrast to mammals and birds, fish display an amazing diversity of genetic sex determination systems, with frequent changes during evolution possibly associated with the emergence of new sex chromosomes and sex-determining genes. To better understand the molecular and evolutionary mechanisms driving this diversity, several fish models are studied in parallel. Besides the medaka (Oryzias latipes Temminck and Schlegel, 1846) for which the master sex-determination gene has been identified, one of the most advanced models for studying sex determination is the Southern platyfish (Xiphophorus maculatus, Günther 1966). Xiphophorus maculatus belongs to the Poeciliids, a family of live-bearing freshwater fish, including platyfish, swordtails and guppies that perfectly illustrates the diversity of genetic sex-determination mechanisms observed in teleosts. For X. maculatus, bacterial artificial chromosome contigs covering the sex-determination region of the X and Y sex chromosomes have been constructed. Initial molecular analysis demonstrated that the sex-determination region is very unstable and frequently undergoes duplications, deletions, inversions and other rearrangements. Eleven gene candidates linked to the master sex-determining gene have been identified, some of them corresponding to pseudogenes. All putative genes are present on both the X and the Y chromosomes, suggesting a poor degree of differentiation and a young evolutionary age for platyfish sex chromosomes. When compared with other fish and tetrapod genomes, syntenies were detected only with autosomes. This observation supports an independent origin of sex chromosomes, not only in different vertebrate lineages but also between different fish species.

Original languageEnglish (US)
Pages (from-to)277-284
Number of pages8
JournalIntegrative zoology
Volume4
Issue number3
DOIs
StatePublished - Sep 1 2009

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sex chromosomes
vertebrates
gender
Y chromosome
fish
genes
Xiphophorus
Oryzias latipes
genetic variation
bacterial artificial chromosomes
Poecilia reticulata
pseudogenes
autosomes
sex determination analysis
Xiphophorus maculatus
freshwater fish
mammals
genome
birds

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Böhne, Astrid ; Schultheis, Christina ; Galiana-Arnoux, Delphine ; Froschauer, Alexander ; Zhou, Qingchun ; Schmidt, Cornelia ; Selz, Yvonne ; Ozouf-Costaz, Catherine ; Dettai, Agnès ; Segurens, Béatrice ; Couloux, Arnaud ; Bernard-Samain, Sylvie ; Barbe, Valérie ; Chilmonczyk, Stefan ; Brunet, Frédéric ; Darras, Amandine ; Hoover, Marta ; Semon, Marie ; Schartl, Manfred ; Volff, Jean Nicolas. / Molecular analysis of the sex chromosomes of the platyfish Xiphophorus maculatus : Towards the identification of a new type of master sexual regulator in vertebrates. In: Integrative zoology. 2009 ; Vol. 4, No. 3. pp. 277-284.
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abstract = "In contrast to mammals and birds, fish display an amazing diversity of genetic sex determination systems, with frequent changes during evolution possibly associated with the emergence of new sex chromosomes and sex-determining genes. To better understand the molecular and evolutionary mechanisms driving this diversity, several fish models are studied in parallel. Besides the medaka (Oryzias latipes Temminck and Schlegel, 1846) for which the master sex-determination gene has been identified, one of the most advanced models for studying sex determination is the Southern platyfish (Xiphophorus maculatus, G{\"u}nther 1966). Xiphophorus maculatus belongs to the Poeciliids, a family of live-bearing freshwater fish, including platyfish, swordtails and guppies that perfectly illustrates the diversity of genetic sex-determination mechanisms observed in teleosts. For X. maculatus, bacterial artificial chromosome contigs covering the sex-determination region of the X and Y sex chromosomes have been constructed. Initial molecular analysis demonstrated that the sex-determination region is very unstable and frequently undergoes duplications, deletions, inversions and other rearrangements. Eleven gene candidates linked to the master sex-determining gene have been identified, some of them corresponding to pseudogenes. All putative genes are present on both the X and the Y chromosomes, suggesting a poor degree of differentiation and a young evolutionary age for platyfish sex chromosomes. When compared with other fish and tetrapod genomes, syntenies were detected only with autosomes. This observation supports an independent origin of sex chromosomes, not only in different vertebrate lineages but also between different fish species.",
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Böhne, A, Schultheis, C, Galiana-Arnoux, D, Froschauer, A, Zhou, Q, Schmidt, C, Selz, Y, Ozouf-Costaz, C, Dettai, A, Segurens, B, Couloux, A, Bernard-Samain, S, Barbe, V, Chilmonczyk, S, Brunet, F, Darras, A, Hoover, M, Semon, M, Schartl, M & Volff, JN 2009, 'Molecular analysis of the sex chromosomes of the platyfish Xiphophorus maculatus: Towards the identification of a new type of master sexual regulator in vertebrates.', Integrative zoology, vol. 4, no. 3, pp. 277-284. https://doi.org/10.1111/j.1749-4877.2009.00166.x

Molecular analysis of the sex chromosomes of the platyfish Xiphophorus maculatus : Towards the identification of a new type of master sexual regulator in vertebrates. / Böhne, Astrid; Schultheis, Christina; Galiana-Arnoux, Delphine; Froschauer, Alexander; Zhou, Qingchun; Schmidt, Cornelia; Selz, Yvonne; Ozouf-Costaz, Catherine; Dettai, Agnès; Segurens, Béatrice; Couloux, Arnaud; Bernard-Samain, Sylvie; Barbe, Valérie; Chilmonczyk, Stefan; Brunet, Frédéric; Darras, Amandine; Hoover, Marta; Semon, Marie; Schartl, Manfred; Volff, Jean Nicolas.

In: Integrative zoology, Vol. 4, No. 3, 01.09.2009, p. 277-284.

Research output: Contribution to journalArticle

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AU - Böhne, Astrid

AU - Schultheis, Christina

AU - Galiana-Arnoux, Delphine

AU - Froschauer, Alexander

AU - Zhou, Qingchun

AU - Schmidt, Cornelia

AU - Selz, Yvonne

AU - Ozouf-Costaz, Catherine

AU - Dettai, Agnès

AU - Segurens, Béatrice

AU - Couloux, Arnaud

AU - Bernard-Samain, Sylvie

AU - Barbe, Valérie

AU - Chilmonczyk, Stefan

AU - Brunet, Frédéric

AU - Darras, Amandine

AU - Hoover, Marta

AU - Semon, Marie

AU - Schartl, Manfred

AU - Volff, Jean Nicolas

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