Molecular and biochemical bases of gametophytic self-incompatibility in solanaceae

T. H. Kao, A. G. McCubbin

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Gametophytic self-incompatibility is a self/nonself recognition mechanism in flowering plants that allows the female reproductive organ to recognize and reject self pollen or pollen from genetically related individuals. For solanaceous species that display gametophytic self-incompatibility, a single multiallelic locus, called the S-locus, controls the outcome of pollination. S-allele-associated proteins (S-proteins) in the pistil have been identified based on their cosegregation with S-alleles. All the characteristics of S-proteins determined are consistent with their playing a role in self-incompatibility. Further, both gain-of-function and loss-of-function approaches have been used to show that S-proteins indeed control the ability of the pistil to recognize and reject self pollen. Since S-proteins have RNase activity and are glycoproteins, site-directed mutagenesis has been carried out to show that the RNase activity, but not the carbohydrate moiety, is essential for the function of S-proteins. Thus, the biochemical mechanism of S-protein-mediated rejection of self pollen involves the cytotoxic action of the RNase activity, and the S-allele specificity determinant of S-proteins lies in their amino acid sequence. Chimeric S-alleles between S1- and S3-alleles of Petunia inflata have been constructed and are being used to identify the S-allele specificity determinant of S-proteins. Models for how S-proteins specifically inhibit self pollen are discussed.

Original languageEnglish (US)
Pages (from-to)171-176
Number of pages6
JournalPlant Physiology and Biochemistry
Volume35
Issue number3
StatePublished - Jan 1 1997

Fingerprint

Solanaceae
Alleles
alleles
Proteins
Pollen
Ribonucleases
pollen
proteins
pistil
Mutagenesis
Petunia integrifolia
Petunia
Glycoproteins
Pollination
loci
Aptitude
biochemical mechanisms
Internal-External Control
Carbohydrates
site-directed mutagenesis

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

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title = "Molecular and biochemical bases of gametophytic self-incompatibility in solanaceae",
abstract = "Gametophytic self-incompatibility is a self/nonself recognition mechanism in flowering plants that allows the female reproductive organ to recognize and reject self pollen or pollen from genetically related individuals. For solanaceous species that display gametophytic self-incompatibility, a single multiallelic locus, called the S-locus, controls the outcome of pollination. S-allele-associated proteins (S-proteins) in the pistil have been identified based on their cosegregation with S-alleles. All the characteristics of S-proteins determined are consistent with their playing a role in self-incompatibility. Further, both gain-of-function and loss-of-function approaches have been used to show that S-proteins indeed control the ability of the pistil to recognize and reject self pollen. Since S-proteins have RNase activity and are glycoproteins, site-directed mutagenesis has been carried out to show that the RNase activity, but not the carbohydrate moiety, is essential for the function of S-proteins. Thus, the biochemical mechanism of S-protein-mediated rejection of self pollen involves the cytotoxic action of the RNase activity, and the S-allele specificity determinant of S-proteins lies in their amino acid sequence. Chimeric S-alleles between S1- and S3-alleles of Petunia inflata have been constructed and are being used to identify the S-allele specificity determinant of S-proteins. Models for how S-proteins specifically inhibit self pollen are discussed.",
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Molecular and biochemical bases of gametophytic self-incompatibility in solanaceae. / Kao, T. H.; McCubbin, A. G.

In: Plant Physiology and Biochemistry, Vol. 35, No. 3, 01.01.1997, p. 171-176.

Research output: Contribution to journalReview article

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AB - Gametophytic self-incompatibility is a self/nonself recognition mechanism in flowering plants that allows the female reproductive organ to recognize and reject self pollen or pollen from genetically related individuals. For solanaceous species that display gametophytic self-incompatibility, a single multiallelic locus, called the S-locus, controls the outcome of pollination. S-allele-associated proteins (S-proteins) in the pistil have been identified based on their cosegregation with S-alleles. All the characteristics of S-proteins determined are consistent with their playing a role in self-incompatibility. Further, both gain-of-function and loss-of-function approaches have been used to show that S-proteins indeed control the ability of the pistil to recognize and reject self pollen. Since S-proteins have RNase activity and are glycoproteins, site-directed mutagenesis has been carried out to show that the RNase activity, but not the carbohydrate moiety, is essential for the function of S-proteins. Thus, the biochemical mechanism of S-protein-mediated rejection of self pollen involves the cytotoxic action of the RNase activity, and the S-allele specificity determinant of S-proteins lies in their amino acid sequence. Chimeric S-alleles between S1- and S3-alleles of Petunia inflata have been constructed and are being used to identify the S-allele specificity determinant of S-proteins. Models for how S-proteins specifically inhibit self pollen are discussed.

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