Molecular aspects of cloricromene (AD6) distribution in human platelets and its pharmacological effects

R. A. Travagli, A. Zatta, N. Banzatto, M. Finesso, R. Mariot, F. Tessari, M. Prosdocimi

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Cloricromene (AD6) is an investigational drug which inhibits platelet aggregation and release reaction. We studied the relationship between its action and its distribution and metabolism in platelets. Incubation of anticoagulated whole blood or platelet-rich plasma (PRP) without exogenous aggregating agents resulted in a progressive decrease of platelet count with a concomitant increase of β-thromboglobulin (BTG) release. AD6 (20-50 μmol/1), but not acetylsalicylic acid (ASA), incubated with whole blood or PRP, prevented the fall in platelet count and the release of BTG for at least 150 min. Moreover, incubation of PRP with AD6 (50 μmol/1) and subsequent stimulation by ADP at threshold concentrations resulted in a significant reduction (about 30%) in aggregation for at least 90 min. AD6 (20 μmol/1) added to PRP was rapidly metabolized by hydrolysis of an ester bond to AD6 acid, a stable catabolite pharmacologically inactive in platelets. Significant amounts of AD6 acid (up to 13.26 ± 2.80 pmol/106 platelets) were associated with the platelets after incubation either at 37°C or 4°C. The amount of AD6 acid in the platelet pellet was proportional to AD6 concentration (2 to 100 μmol/1). PRP incubation with AD6 acid (20 μmol/1) resulted in very low levels (< 1 pmol/106 platelets) of the same compound in the platelet pellet after 1, 5 or 30 min. These data suggest that AD6 is taken up as an ester and converted to its acid catabolite with a consequent long-lasting inhibition of platelet function.

Original languageEnglish (US)
Pages (from-to)327-338
Number of pages12
JournalThrombosis Research
Volume54
Issue number4
DOIs
StatePublished - May 15 1989

All Science Journal Classification (ASJC) codes

  • Hematology

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