Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages217-230
Number of pages14
Volume1110
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)10643745

Fingerprint

Meiosis
Arabidopsis
Cell Biology
Molecular Biology
Chromosomes
Cellular Structures
Fluorescence In Situ Hybridization
Fluorescence Microscopy
Electron Microscopy
Electrons
Technology
Light

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Wang, Y., Cheng, Z., Lu, P., Timofejeva, L., & Ma, H. (2014). Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. In Methods in Molecular Biology (Vol. 1110, pp. 217-230). (Methods in Molecular Biology; Vol. 1110). https://doi.org/10.1007/978-1-4614-9408-9-10
Wang, Yingxiang ; Cheng, Zhihao ; Lu, Pingli ; Timofejeva, Ljudmilla ; Ma, Hong. / Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. Methods in Molecular Biology. Vol. 1110 2014. pp. 217-230 (Methods in Molecular Biology).
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Wang, Y, Cheng, Z, Lu, P, Timofejeva, L & Ma, H 2014, Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. in Methods in Molecular Biology. vol. 1110, Methods in Molecular Biology, vol. 1110, pp. 217-230. https://doi.org/10.1007/978-1-4614-9408-9-10

Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. / Wang, Yingxiang; Cheng, Zhihao; Lu, Pingli; Timofejeva, Ljudmilla; Ma, Hong.

Methods in Molecular Biology. Vol. 1110 2014. p. 217-230 (Methods in Molecular Biology; Vol. 1110).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Wang Y, Cheng Z, Lu P, Timofejeva L, Ma H. Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. In Methods in Molecular Biology. Vol. 1110. 2014. p. 217-230. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4614-9408-9-10