Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages217-230
Number of pages14
Volume1110
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)10643745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana'. Together they form a unique fingerprint.

  • Cite this

    Wang, Y., Cheng, Z., Lu, P., Timofejeva, L., & Ma, H. (2014). Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. In Methods in Molecular Biology (Vol. 1110, pp. 217-230). (Methods in Molecular Biology; Vol. 1110). https://doi.org/10.1007/978-1-4614-9408-9-10