Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.

Original languageEnglish (US)
Title of host publicationFlower Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages217-230
Number of pages14
ISBN (Print)9781461494072
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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