Molecular characterization of a novel putative partitivirus infecting Cytospora sacchari, a plant pathogenic fungus

Mahtab Peyambari, Mina Koohi Habibi, Khalil Berdi Fotouhifar, Akbar Dizadji, Marilyn J. Roossinck

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Three double-stranded RNAs (dsRNAs), approximately 1.85, 1.65 and 1.27 kb in size, were detected in an isolate of Cytospora sacchari from Iran. Partial nucleotide sequence revealed a 1,284 bp segment containing one ORF that potentially encodes a 405 aa protein. This protein contains conserved motifs related to RNA dependent RNA polymerases (RdRp) that showed similarity to RdRps of partitiviruses. The results indicate that these dsRNAs represent a novel Partitivirus that we tentatively designate Cytospora sacchari partitivirus (CsPV). Treatment of the fungal strain by cyclohexamide and also hyphal tip culture had no effect on removing the putative virus. Phylogenetic analysis of putative RdRp of CsPV and other partitiviruses places CsPV as a member of the genus Partitivirus in the family Partitiviridae, and clustering with Aspergillus ochraceous virus 1.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalPlant Pathology Journal
Volume30
Issue number2
DOIs
StatePublished - Jan 1 2014

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Partitivirus
Cytospora
Partitiviridae
plant pathogenic fungi
RNA-directed RNA polymerase
double-stranded RNA
open reading frames
Iran
proteins
nucleotide sequences
viruses
phylogeny

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Peyambari, Mahtab ; Habibi, Mina Koohi ; Fotouhifar, Khalil Berdi ; Dizadji, Akbar ; Roossinck, Marilyn J. / Molecular characterization of a novel putative partitivirus infecting Cytospora sacchari, a plant pathogenic fungus. In: Plant Pathology Journal. 2014 ; Vol. 30, No. 2. pp. 151-158.
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Molecular characterization of a novel putative partitivirus infecting Cytospora sacchari, a plant pathogenic fungus. / Peyambari, Mahtab; Habibi, Mina Koohi; Fotouhifar, Khalil Berdi; Dizadji, Akbar; Roossinck, Marilyn J.

In: Plant Pathology Journal, Vol. 30, No. 2, 01.01.2014, p. 151-158.

Research output: Contribution to journalArticle

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