Molecular cloning and characterization of GPA1, a G protein α subunit gene from Arabidopsis thaliana

Hong Ma, M. F. Yanofsky, E. M. Meyerowitz

Research output: Contribution to journalArticle

270 Citations (Scopus)

Abstract

We have isolated a gene coding for a G protein α subunit from the flowering plant Arabidopsis thaliana. This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein α subunits. The sequences of genomic and cDNA clones indicate that GPA1 has 14 exons, and the deduced amino acid sequence shows that the GPA1 gene product (GPα1) has 383 amino acid residues (44,582 Da). The GPα1 protein exhibits similarity to all known G protein α subunits - 36% of its amino acids are identical and 73% are similar (identical and conservative changes) to mammalian inhibitory guanine nucleotide-binding regulatory factor α subunits and transducins. Furthermore, the GPα1 protein has all of the consensus regions for a GTP-binding protein. The GPA1-encoded mRNA of 1.55 kilobases is most abundant in vegetative plant tissues, as determined by RNA blot analysis. Restriction fragment length polymorphism mapping experiments show that GPA1 is ≃ 1.2 centimorgans from the visible marker er on chromosome 2.

Original languageEnglish (US)
Pages (from-to)3821-3825
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number10
DOIs
StatePublished - Jun 18 1990

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Protein Subunits
Molecular Cloning
GTP-Binding Proteins
Arabidopsis
Genes
Transducin
Amino Acids
Proteins
Fungal Proteins
Chromosomes, Human, Pair 2
Guanine Nucleotides
DNA Probes
Restriction Fragment Length Polymorphisms
Amino Acid Sequence
Exons
Complementary DNA
Clone Cells
RNA
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Molecular cloning and characterization of GPA1, a G protein α subunit gene from Arabidopsis thaliana",
abstract = "We have isolated a gene coding for a G protein α subunit from the flowering plant Arabidopsis thaliana. This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein α subunits. The sequences of genomic and cDNA clones indicate that GPA1 has 14 exons, and the deduced amino acid sequence shows that the GPA1 gene product (GPα1) has 383 amino acid residues (44,582 Da). The GPα1 protein exhibits similarity to all known G protein α subunits - 36{\%} of its amino acids are identical and 73{\%} are similar (identical and conservative changes) to mammalian inhibitory guanine nucleotide-binding regulatory factor α subunits and transducins. Furthermore, the GPα1 protein has all of the consensus regions for a GTP-binding protein. The GPA1-encoded mRNA of 1.55 kilobases is most abundant in vegetative plant tissues, as determined by RNA blot analysis. Restriction fragment length polymorphism mapping experiments show that GPA1 is ≃ 1.2 centimorgans from the visible marker er on chromosome 2.",
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Molecular cloning and characterization of GPA1, a G protein α subunit gene from Arabidopsis thaliana. / Ma, Hong; Yanofsky, M. F.; Meyerowitz, E. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 10, 18.06.1990, p. 3821-3825.

Research output: Contribution to journalArticle

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AB - We have isolated a gene coding for a G protein α subunit from the flowering plant Arabidopsis thaliana. This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein α subunits. The sequences of genomic and cDNA clones indicate that GPA1 has 14 exons, and the deduced amino acid sequence shows that the GPA1 gene product (GPα1) has 383 amino acid residues (44,582 Da). The GPα1 protein exhibits similarity to all known G protein α subunits - 36% of its amino acids are identical and 73% are similar (identical and conservative changes) to mammalian inhibitory guanine nucleotide-binding regulatory factor α subunits and transducins. Furthermore, the GPα1 protein has all of the consensus regions for a GTP-binding protein. The GPA1-encoded mRNA of 1.55 kilobases is most abundant in vegetative plant tissues, as determined by RNA blot analysis. Restriction fragment length polymorphism mapping experiments show that GPA1 is ≃ 1.2 centimorgans from the visible marker er on chromosome 2.

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