Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus

R. H. Ffrench-Constant, D. P. Mortlock, C. D. Shaffer, R. J. MacIntyre, Richard T. Roush

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the γ-aminobutyric acid subtype A (GABA(A)) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a 'chromosomal walk' and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABA(A) subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.

Original languageEnglish (US)
Pages (from-to)7209-7213
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number16
DOIs
StatePublished - Jan 1 1991

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Aminobutyrates
Molecular Cloning
Invertebrates
Germ Cells
Drosophila
Insects
Vertebrates
Organism Cloning
Insecticide Resistance
GABA Receptors
Ionophores
Protein Sequence Analysis
GABA-A Receptors
gamma-Aminobutyric Acid
Chlorides
Complementary DNA
Binding Sites
Phenotype
Genes
picrotoxinin

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus",
abstract = "Cyclodiene resistance represents 60{\%} of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the γ-aminobutyric acid subtype A (GABA(A)) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a 'chromosomal walk' and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABA(A) subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.",
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Molecular cloning and transformation of cyclodiene resistance in Drosophila : An invertebrate γ-aminobutyric acid subtype A receptor locus. / Ffrench-Constant, R. H.; Mortlock, D. P.; Shaffer, C. D.; MacIntyre, R. J.; Roush, Richard T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 16, 01.01.1991, p. 7209-7213.

Research output: Contribution to journalArticle

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T2 - An invertebrate γ-aminobutyric acid subtype A receptor locus

AU - Ffrench-Constant, R. H.

AU - Mortlock, D. P.

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AU - MacIntyre, R. J.

AU - Roush, Richard T.

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