Molecular cloning of a member of the facilitative glucose transporter gene family GLUT11 (SLC2A11) and identification of transcription variants

Takashi Sasaki, Shinsei Minoshima, Aiko Shiohama, Ai Shintani, Atsushi Shimizu, Shuichi Asakawa, Kazuhiko Kawasaki, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We isolated a member of the facilitative glucose transporter (GLUT) gene family (GLUT11; SLC2A11 as a HGMW-approved symbol) based on the analysis of a human genomic BAC clone KB1125A3 located on band q11.2 of human chromosome 22. The gene GLUT11/SLC2A11 consists of 12 exons spanning over 29 kb in size and is located between two genes, SMARCB1 and MIF. The deduced amino acid sequence indicated the topological features of transmembrane helices and sequence motifs which are common to the GLUT protein family. The cDNA cloning revealed the presence of three types of variation in its transcripts. The first variation is caused by the existence of three distinct first exons (SLC2A11-a, -b, and -c). PCR analysis of multi-tissue -derived cDNA panels indicated the differential expression of these transcript variants. The second variation is caused by skipping over one exon (exon 6). The third variation is caused by the premature transcription termination at a site between exon 8 and exon 9. Both exon skipping and premature termination caused frameshift, resulting in the production of truncated GLUT11/SLC2A11 transcripts. These results suggested that transcription of GLUT11/SCL2A11 gene is controlled in a complex manner.

Original languageEnglish (US)
Pages (from-to)1218-1224
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number5
DOIs
StatePublished - Dec 21 2001

Fingerprint

Facilitative Glucose Transport Proteins
Cloning
Molecular Cloning
Transcription
Exons
Genes
Complementary DNA
Chromosomes, Human, Pair 22
Human Chromosomes
Chromosomes
Organism Cloning
Amino Acid Sequence
Clone Cells
Tissue
Amino Acids
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sasaki, Takashi ; Minoshima, Shinsei ; Shiohama, Aiko ; Shintani, Ai ; Shimizu, Atsushi ; Asakawa, Shuichi ; Kawasaki, Kazuhiko ; Shimizu, Nobuyoshi. / Molecular cloning of a member of the facilitative glucose transporter gene family GLUT11 (SLC2A11) and identification of transcription variants. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 289, No. 5. pp. 1218-1224.
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Molecular cloning of a member of the facilitative glucose transporter gene family GLUT11 (SLC2A11) and identification of transcription variants. / Sasaki, Takashi; Minoshima, Shinsei; Shiohama, Aiko; Shintani, Ai; Shimizu, Atsushi; Asakawa, Shuichi; Kawasaki, Kazuhiko; Shimizu, Nobuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 289, No. 5, 21.12.2001, p. 1218-1224.

Research output: Contribution to journalArticle

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AU - Shimizu, Atsushi

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AU - Kawasaki, Kazuhiko

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