Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata

Vladimir Vincek, Dagmar Klein, Robert T. Graser, Felipe Figueroa, Colm O'hUigin, Jan Klein

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The only avian major histocompatibility complex (Mhc) genes thus far identified are from species of the relatively small order of Galliformes, while by far the largest order of Passeriformes (songbirds), containing some 60% of extant bird species, has not been studied at all in this regard. The Galliformes emerged more than 55 million years (my) ago, the Passeriformes some 25 my ago. Because of the potential for the use of Mhc genes as markers in the study of songbird populations, an attempt was made to clone class II B genes of a passeriform species, the Bangalese finch Lonchura striata acuticauda. Using a set of primers designed on the basis of known sequences, a probe corresponding to part of exon II was obtained by the polymerase chain reaction. The probe was then used to screen a Bengalese finch cDNA library and to isolate and sequence two nearly full-length clones. The sequences reveal the presence of one presumably functional class II B locus in this bird species.

Original languageEnglish (US)
Pages (from-to)262-267
Number of pages6
JournalImmunogenetics
Volume42
Issue number4
DOIs
StatePublished - Jan 1 1995

Fingerprint

Galliformes
Passeriformes
Finches
MHC Class II Genes
Songbirds
Molecular Cloning
Major Histocompatibility Complex
Birds
Complementary DNA
Clone Cells
Gene Library
Genes
Exons
Polymerase Chain Reaction
Population

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Vincek, Vladimir ; Klein, Dagmar ; Graser, Robert T. ; Figueroa, Felipe ; O'hUigin, Colm ; Klein, Jan. / Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata. In: Immunogenetics. 1995 ; Vol. 42, No. 4. pp. 262-267.
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Vincek, V, Klein, D, Graser, RT, Figueroa, F, O'hUigin, C & Klein, J 1995, 'Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata', Immunogenetics, vol. 42, no. 4, pp. 262-267. https://doi.org/10.1007/BF00176443

Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata. / Vincek, Vladimir; Klein, Dagmar; Graser, Robert T.; Figueroa, Felipe; O'hUigin, Colm; Klein, Jan.

In: Immunogenetics, Vol. 42, No. 4, 01.01.1995, p. 262-267.

Research output: Contribution to journalArticle

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