Molecular cloning of the mouse ouabain-resistance gene

R. Levenson, V. Racaniello, L. Albritton, D. Housman

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

DNA prepared from ouabain-resistant mouse cells was able to transform ouabain-sensitive CV-1 cells to ouabain resistance after DNA-mediated gene transfer. The murine DNA fragment responsible for ouabain resistance was detected on the background of CV-1 DNA by virtue of a repetitive DNA sequence element that reacts positively with a mouse repeat DNA clone. CV-1 DNA is nonreactive with this probe. Southern analysis of several independently derived ouabain-resistant transformants indicates that the mouse oua(R) gene is located on a 6.5-kilobase EcoRI restriction fragment. The 6.5-kilobase DNA fragment was initially isolated from a λ phage library made from a ouabain-resistant secondary transformant and subsequently was subcloned in the plasmid vector pAT153. This plasmid was able to transform wild-type CV-1 cells to ouabain resistance at a frequency of about 10 cells per ng of DNA.

Original languageEnglish (US)
Pages (from-to)1489-1493
Number of pages5
JournalISOTOPENPRAXIS
Volume20
Issue number1
StatePublished - 1984

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Levenson, R., Racaniello, V., Albritton, L., & Housman, D. (1984). Molecular cloning of the mouse ouabain-resistance gene. ISOTOPENPRAXIS, 20(1), 1489-1493.