Molecular cloning of the mouse ouabain-resistance gene

Robert Levenson, V. Racaniello, L. Albritton, D. Housman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

DNA prepared from ouabain-resistant mouse cells was able to transform ouabain-sensitive CV-1 cells to ouabain resistance after DNA-mediated gene transfer. The murine DNA fragment responsible for ouabain resistance was detected on the background of CV-1 DNA by virtue of a repetitive DNA sequence element that reacts positively with a mouse repeat DNA clone. CV-1 DNA is nonreactive with this probe. Southern analysis of several independently derived ouabain-resistant transformants indicates that the mouse oua(R) gene is located on a 6.5-kilobase EcoRI restriction fragment. The 6.5-kilobase DNA fragment was initially isolated from a λ phage library made from a ouabain-resistant secondary transformant and subsequently was subcloned in the plasmid vector pAT153. This plasmid was able to transform wild-type CV-1 cells to ouabain resistance at a frequency of about 10 cells per ng of DNA.

Original languageEnglish (US)
Pages (from-to)1489-1493
Number of pages5
JournalIsotopenpraxis
Volume20
Issue number1
StatePublished - Jan 1 1984

Fingerprint

Cloning
Molecular Cloning
Ouabain
Genes
DNA
gene
plasmid
Plasmids
transform
vpr Genes
Gene transfer
Bacteriophages
cloning
Nucleic Acid Repetitive Sequences
DNA sequences
gene transfer
Clone Cells
clone
probe
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Levenson, R., Racaniello, V., Albritton, L., & Housman, D. (1984). Molecular cloning of the mouse ouabain-resistance gene. Isotopenpraxis, 20(1), 1489-1493.
Levenson, Robert ; Racaniello, V. ; Albritton, L. ; Housman, D. / Molecular cloning of the mouse ouabain-resistance gene. In: Isotopenpraxis. 1984 ; Vol. 20, No. 1. pp. 1489-1493.
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Levenson, R, Racaniello, V, Albritton, L & Housman, D 1984, 'Molecular cloning of the mouse ouabain-resistance gene', Isotopenpraxis, vol. 20, no. 1, pp. 1489-1493.

Molecular cloning of the mouse ouabain-resistance gene. / Levenson, Robert; Racaniello, V.; Albritton, L.; Housman, D.

In: Isotopenpraxis, Vol. 20, No. 1, 01.01.1984, p. 1489-1493.

Research output: Contribution to journalArticle

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AB - DNA prepared from ouabain-resistant mouse cells was able to transform ouabain-sensitive CV-1 cells to ouabain resistance after DNA-mediated gene transfer. The murine DNA fragment responsible for ouabain resistance was detected on the background of CV-1 DNA by virtue of a repetitive DNA sequence element that reacts positively with a mouse repeat DNA clone. CV-1 DNA is nonreactive with this probe. Southern analysis of several independently derived ouabain-resistant transformants indicates that the mouse oua(R) gene is located on a 6.5-kilobase EcoRI restriction fragment. The 6.5-kilobase DNA fragment was initially isolated from a λ phage library made from a ouabain-resistant secondary transformant and subsequently was subcloned in the plasmid vector pAT153. This plasmid was able to transform wild-type CV-1 cells to ouabain resistance at a frequency of about 10 cells per ng of DNA.

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Levenson R, Racaniello V, Albritton L, Housman D. Molecular cloning of the mouse ouabain-resistance gene. Isotopenpraxis. 1984 Jan 1;20(1):1489-1493.