Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale

Darrell W. Cockburn, Nicole I. Orlovsky, Matthew H. Foley, Kurt J. Kwiatkowski, Constance M. Bahr, Mallory Maynard, Borries Demeler, Nicole M. Koropatkin

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Summary: Eubacterium rectale is a prominent human gut symbiont yet little is known about the molecular strategies this bacterium has developed to acquire nutrients within the competitive gut ecosystem. Starch is one of the most abundant glycans in the human diet, and E.rectale increases in vivo when the host consumes a diet rich in resistant starch, although it is not a primary degrader of this glycan. Here we present the results of a quantitative proteomics study in which we identify two glycoside hydrolase 13 family enzymes, and three ABC transporter solute-binding proteins that are abundant during growth on starch and, we hypothesize, work together at the cell surface to degrade starch and capture the released maltooligosaccharides. EUR_21100 is a multidomain cell wall anchored amylase that preferentially targets starch polysaccharides, liberating maltotetraose, whereas the membrane-associated maltogenic amylase EUR_01860 breaks down maltooligosaccharides longer than maltotriose. The three solute-binding proteins display a range of glycan-binding specificities that ensure the capture of glucose through maltoheptaose and some α1,6-branched glycans. Taken together, we describe a pathway for starch utilization by E.rectaleDSM 17629 that may be conserved among other starch-degrading Clostridium cluster XIVa organisms in the human gut.

Original languageEnglish (US)
Pages (from-to)209-230
Number of pages22
JournalMolecular Microbiology
Volume95
Issue number2
DOIs
StatePublished - Jan 1 2015

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Eubacterium
Starch
Polysaccharides
glucan 1,4-alpha-maltohydrolase
Carrier Proteins
Diet
ATP-Binding Cassette Transporters
Clostridium
Glycoside Hydrolases
Amylases
Proteomics
Cell Wall
Ecosystem
Bacteria
Glucose
Food
Membranes
Enzymes
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Cockburn, D. W., Orlovsky, N. I., Foley, M. H., Kwiatkowski, K. J., Bahr, C. M., Maynard, M., ... Koropatkin, N. M. (2015). Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Molecular Microbiology, 95(2), 209-230. https://doi.org/10.1111/mmi.12859
Cockburn, Darrell W. ; Orlovsky, Nicole I. ; Foley, Matthew H. ; Kwiatkowski, Kurt J. ; Bahr, Constance M. ; Maynard, Mallory ; Demeler, Borries ; Koropatkin, Nicole M. / Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. In: Molecular Microbiology. 2015 ; Vol. 95, No. 2. pp. 209-230.
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Cockburn, DW, Orlovsky, NI, Foley, MH, Kwiatkowski, KJ, Bahr, CM, Maynard, M, Demeler, B & Koropatkin, NM 2015, 'Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale', Molecular Microbiology, vol. 95, no. 2, pp. 209-230. https://doi.org/10.1111/mmi.12859

Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. / Cockburn, Darrell W.; Orlovsky, Nicole I.; Foley, Matthew H.; Kwiatkowski, Kurt J.; Bahr, Constance M.; Maynard, Mallory; Demeler, Borries; Koropatkin, Nicole M.

In: Molecular Microbiology, Vol. 95, No. 2, 01.01.2015, p. 209-230.

Research output: Contribution to journalArticle

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T1 - Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale

AU - Cockburn, Darrell W.

AU - Orlovsky, Nicole I.

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AU - Bahr, Constance M.

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AU - Demeler, Borries

AU - Koropatkin, Nicole M.

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