Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes

Reza Riahi, Kathleen E. MacH, Ruchika Mohan, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Rapid, specific, and sensitive detection of bacterial pathogens is essential toward clinical management of infectious diseases. Traditional approaches for pathogen detection, however, often require time-intensive bacterial culture and amplification procedures. Herein, a microparticle enhanced double-stranded DNA probe is demonstrated for rapid species-specific detection of bacterial 16S rRNA. In this molecular assay, the binding of the target sequence to the fluorophore conjugated probe thermodynamically displaces the quencher probe and allows the fluorophore to fluoresce. By incorporation of streptavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay can be improved by 3 orders of magnitude. The limit of detection of the assay is as few as eight bacteria without target amplification and is highly specific against other common pathogens. Its applicability toward clinical diagnostics is demonstrated by directly identifying bacterial pathogens in urine samples from patients with urinary tract infections.

Original languageEnglish (US)
Pages (from-to)6349-6354
Number of pages6
JournalAnalytical chemistry
Volume83
Issue number16
DOIs
StatePublished - Aug 15 2011

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes'. Together they form a unique fingerprint.

Cite this