Molecular evolution of inversions in Drosophila pseudoobscura: The amylase gene region

C. F. Aquadro, A. L. Weaver, Stephen Wade Schaeffer, W. W. Anderson

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a dendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number1
DOIs
StatePublished - Jan 1 1991

Fingerprint

Gene Order
Molecular Evolution
Amylases
Drosophila
Chromosomes
Genes
Chromosome Deletion
Phylogeny
Cytogenetics
Haplotypes
Cluster Analysis
Clone Cells
DNA
Population

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{c6234d3e3a4d43ff9b3b913b595d9617,
title = "Molecular evolution of inversions in Drosophila pseudoobscura: The amylase gene region",
abstract = "The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a dendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old.",
author = "Aquadro, {C. F.} and Weaver, {A. L.} and Schaeffer, {Stephen Wade} and Anderson, {W. W.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1073/pnas.88.1.305",
language = "English (US)",
volume = "88",
pages = "305--309",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "1",

}

Molecular evolution of inversions in Drosophila pseudoobscura : The amylase gene region. / Aquadro, C. F.; Weaver, A. L.; Schaeffer, Stephen Wade; Anderson, W. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 1, 01.01.1991, p. 305-309.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular evolution of inversions in Drosophila pseudoobscura

T2 - The amylase gene region

AU - Aquadro, C. F.

AU - Weaver, A. L.

AU - Schaeffer, Stephen Wade

AU - Anderson, W. W.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a dendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old.

AB - The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a dendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old.

UR - http://www.scopus.com/inward/record.url?scp=0026007855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026007855&partnerID=8YFLogxK

U2 - 10.1073/pnas.88.1.305

DO - 10.1073/pnas.88.1.305

M3 - Article

C2 - 1702542

AN - SCOPUS:0026007855

VL - 88

SP - 305

EP - 309

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 1

ER -