Molecular genetic mapping of the plum pox virus resistance genes in apricot

D. Abernathy, Tetyana Zhebentyayeva, A. G. Abbott, S. Vilanova, M. L. Badenes, J. Salava, J. Polák, B. Krska, V. D. Damsteegt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

Plum pox virus (PPV) is a devastating disease of Prunus trees such as apricot, peach, plum, nectarine and cherry. It is the most economically important disease of fruit crops in Europe and as of 1999 has found its way into the United States. Development of molecular genetic markers associated with disease resistance in Prunus species could prove to be a valuable tool in combating disease caused by PPV. In order to do this, a molecular genetic approach has been initiated to map putative resistance regions associated with resistance in apricot. Four dominant AFLP markers segregating with resistance to PPV have been previously mapped in the crosses 'Stark Early Orange' (resistant) × 'LE-3218' (a susceptible selection) and 'Vestar' (susceptible) x 'LE-3246' (a resistant selection). The markers EAA/MCAG, EAG/MCAT, EAT/MCCT, and ETC/MCCT are located 4.5, 4.5, 13.3, and 16.4 cM respectively from the putative resistance gene. In order to further characterize this region, each marker was cloned, sequenced and hybridized to an apricot BAC library. Eleven apricot BACs were positively identified as containing a specific AFLP marker fragment. Based on Hind III digestion patterns of these BACs, 6 of the 11 BACs were chosen for sub-cloning and hybridized to the SSR (simple sequence repeats) oligonucleotide probes. Sub-clones that hybridized to the oligonucleotide probes were sequenced in order to develop SSRs in this region. Eight SSRs identified are currently being investigated to determine their potential for mapping and screening in other crosses. Due to the co-dominant nature of SSRs and their high genetic transportability, development of SSRs linked to resistance to PPV in apricot could allow the plant breeder to use MAS (marker assisted selection) to screen resistant cultivars in apricot and potentially in other Prunus species.

Original languageEnglish (US)
Title of host publicationXIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
PublisherInternational Society for Horticultural Science
Pages283-288
Number of pages6
ISBN (Print)9789066051485
DOIs
StatePublished - Sep 25 2004

Publication series

NameActa Horticulturae
Volume657
ISSN (Print)0567-7572

Fingerprint

Plum pox virus
apricots
molecular genetics
chromosome mapping
Prunus
oligonucleotide probes
genes
amplified fragment length polymorphism
nectarines
plant breeders
fruit crops
plums
marker-assisted selection
peaches
disease resistance
molecular cloning
digestion
microsatellite repeats
clones
screening

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Abernathy, D., Zhebentyayeva, T., Abbott, A. G., Vilanova, S., Badenes, M. L., Salava, J., ... Damsteegt, V. D. (2004). Molecular genetic mapping of the plum pox virus resistance genes in apricot. In XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases (pp. 283-288). (Acta Horticulturae; Vol. 657). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2004.657.43
Abernathy, D. ; Zhebentyayeva, Tetyana ; Abbott, A. G. ; Vilanova, S. ; Badenes, M. L. ; Salava, J. ; Polák, J. ; Krska, B. ; Damsteegt, V. D. / Molecular genetic mapping of the plum pox virus resistance genes in apricot. XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases. International Society for Horticultural Science, 2004. pp. 283-288 (Acta Horticulturae).
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abstract = "Plum pox virus (PPV) is a devastating disease of Prunus trees such as apricot, peach, plum, nectarine and cherry. It is the most economically important disease of fruit crops in Europe and as of 1999 has found its way into the United States. Development of molecular genetic markers associated with disease resistance in Prunus species could prove to be a valuable tool in combating disease caused by PPV. In order to do this, a molecular genetic approach has been initiated to map putative resistance regions associated with resistance in apricot. Four dominant AFLP markers segregating with resistance to PPV have been previously mapped in the crosses 'Stark Early Orange' (resistant) × 'LE-3218' (a susceptible selection) and 'Vestar' (susceptible) x 'LE-3246' (a resistant selection). The markers EAA/MCAG, EAG/MCAT, EAT/MCCT, and ETC/MCCT are located 4.5, 4.5, 13.3, and 16.4 cM respectively from the putative resistance gene. In order to further characterize this region, each marker was cloned, sequenced and hybridized to an apricot BAC library. Eleven apricot BACs were positively identified as containing a specific AFLP marker fragment. Based on Hind III digestion patterns of these BACs, 6 of the 11 BACs were chosen for sub-cloning and hybridized to the SSR (simple sequence repeats) oligonucleotide probes. Sub-clones that hybridized to the oligonucleotide probes were sequenced in order to develop SSRs in this region. Eight SSRs identified are currently being investigated to determine their potential for mapping and screening in other crosses. Due to the co-dominant nature of SSRs and their high genetic transportability, development of SSRs linked to resistance to PPV in apricot could allow the plant breeder to use MAS (marker assisted selection) to screen resistant cultivars in apricot and potentially in other Prunus species.",
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Abernathy, D, Zhebentyayeva, T, Abbott, AG, Vilanova, S, Badenes, ML, Salava, J, Polák, J, Krska, B & Damsteegt, VD 2004, Molecular genetic mapping of the plum pox virus resistance genes in apricot. in XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases. Acta Horticulturae, vol. 657, International Society for Horticultural Science, pp. 283-288. https://doi.org/10.17660/ActaHortic.2004.657.43

Molecular genetic mapping of the plum pox virus resistance genes in apricot. / Abernathy, D.; Zhebentyayeva, Tetyana; Abbott, A. G.; Vilanova, S.; Badenes, M. L.; Salava, J.; Polák, J.; Krska, B.; Damsteegt, V. D.

XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases. International Society for Horticultural Science, 2004. p. 283-288 (Acta Horticulturae; Vol. 657).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Zhebentyayeva, Tetyana

AU - Abbott, A. G.

AU - Vilanova, S.

AU - Badenes, M. L.

AU - Salava, J.

AU - Polák, J.

AU - Krska, B.

AU - Damsteegt, V. D.

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N2 - Plum pox virus (PPV) is a devastating disease of Prunus trees such as apricot, peach, plum, nectarine and cherry. It is the most economically important disease of fruit crops in Europe and as of 1999 has found its way into the United States. Development of molecular genetic markers associated with disease resistance in Prunus species could prove to be a valuable tool in combating disease caused by PPV. In order to do this, a molecular genetic approach has been initiated to map putative resistance regions associated with resistance in apricot. Four dominant AFLP markers segregating with resistance to PPV have been previously mapped in the crosses 'Stark Early Orange' (resistant) × 'LE-3218' (a susceptible selection) and 'Vestar' (susceptible) x 'LE-3246' (a resistant selection). The markers EAA/MCAG, EAG/MCAT, EAT/MCCT, and ETC/MCCT are located 4.5, 4.5, 13.3, and 16.4 cM respectively from the putative resistance gene. In order to further characterize this region, each marker was cloned, sequenced and hybridized to an apricot BAC library. Eleven apricot BACs were positively identified as containing a specific AFLP marker fragment. Based on Hind III digestion patterns of these BACs, 6 of the 11 BACs were chosen for sub-cloning and hybridized to the SSR (simple sequence repeats) oligonucleotide probes. Sub-clones that hybridized to the oligonucleotide probes were sequenced in order to develop SSRs in this region. Eight SSRs identified are currently being investigated to determine their potential for mapping and screening in other crosses. Due to the co-dominant nature of SSRs and their high genetic transportability, development of SSRs linked to resistance to PPV in apricot could allow the plant breeder to use MAS (marker assisted selection) to screen resistant cultivars in apricot and potentially in other Prunus species.

AB - Plum pox virus (PPV) is a devastating disease of Prunus trees such as apricot, peach, plum, nectarine and cherry. It is the most economically important disease of fruit crops in Europe and as of 1999 has found its way into the United States. Development of molecular genetic markers associated with disease resistance in Prunus species could prove to be a valuable tool in combating disease caused by PPV. In order to do this, a molecular genetic approach has been initiated to map putative resistance regions associated with resistance in apricot. Four dominant AFLP markers segregating with resistance to PPV have been previously mapped in the crosses 'Stark Early Orange' (resistant) × 'LE-3218' (a susceptible selection) and 'Vestar' (susceptible) x 'LE-3246' (a resistant selection). The markers EAA/MCAG, EAG/MCAT, EAT/MCCT, and ETC/MCCT are located 4.5, 4.5, 13.3, and 16.4 cM respectively from the putative resistance gene. In order to further characterize this region, each marker was cloned, sequenced and hybridized to an apricot BAC library. Eleven apricot BACs were positively identified as containing a specific AFLP marker fragment. Based on Hind III digestion patterns of these BACs, 6 of the 11 BACs were chosen for sub-cloning and hybridized to the SSR (simple sequence repeats) oligonucleotide probes. Sub-clones that hybridized to the oligonucleotide probes were sequenced in order to develop SSRs in this region. Eight SSRs identified are currently being investigated to determine their potential for mapping and screening in other crosses. Due to the co-dominant nature of SSRs and their high genetic transportability, development of SSRs linked to resistance to PPV in apricot could allow the plant breeder to use MAS (marker assisted selection) to screen resistant cultivars in apricot and potentially in other Prunus species.

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Abernathy D, Zhebentyayeva T, Abbott AG, Vilanova S, Badenes ML, Salava J et al. Molecular genetic mapping of the plum pox virus resistance genes in apricot. In XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases. International Society for Horticultural Science. 2004. p. 283-288. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2004.657.43