Molecular linkage maps of the Populus genome

Tongming Yin, Xinye Zhang, Minren Huang, Minxiu Wang, Qiang Zhuge, Shengming Tu, Li Huang Zhu, Rongling Wu

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We report molecular genetic linkage maps for an interspecific hybrid population of Populus, a model system in forest-tree biology. The hybrids were produced by crosses between P. deltoides (mother) and P. euramericana (father), which is a natural hybrid of P. deltoides (grandmother) and P. nigra (grandfather). Linkage analysis from 93 of the 450 backcross progeny grown in the field for 15 years was performed using random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), and inter-simple sequence repeats (ISSRs). Of a total of 839 polymorphic markers identified, 560 (67%) were testcross markers heterozygous in one parent but null in the other (segregating 1:1), 206 (25%) were intercross dominant markers heterozygous in both parents (segregating 3:1), and the remaining 73 (9%) were 19 non-parental RAPD markers (segregating 1:1) and 54 codominant AFLP markers (segregating 1:1:1:1). A mixed set of the testcross markers, non-parental RAPD markers, and codominant AFLP markers was used to construct two linkage maps, one based on the P. deltoides (D) genome and the other based on P. euramericana (E). The two maps showed nearly complete coverage of the genome, spanning 3801 and 3452 cM, respectively. The availability of non-parental RAPD and codominant AFLP markers as orthologous genes allowed for a direct comparison of the rate of meiotic recombination between the two different parental species. Generally, the rate of meiotic recombination was greater for males than females in our interspecific poplar hybrids. The confounded effect of sexes and species causes the mean recombination distance of orthologous markers to be 11% longer for the father (P. euramericana; interspecific hybrid) than for the mother (P. deltoides; pure species). The linkage maps constructed and the interspecific poplar hybrid population in which clonal replicates for individual genotypes are available present a comprehensive foundation for future genomic studies and quantitative trait locus (QTL) identification.

Original languageEnglish (US)
Pages (from-to)541-555
Number of pages15
JournalGenome
Volume45
Issue number3
DOIs
StatePublished - May 8 2002

Fingerprint

Populus
Genome
Genetic Recombination
Genetic Markers
Fathers
Mothers
Genetic Linkage
Quantitative Trait Loci
DNA
Microsatellite Repeats
Population
Molecular Biology
Parents
Genotype
Genes
Grandparents

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Yin, T., Zhang, X., Huang, M., Wang, M., Zhuge, Q., Tu, S., ... Wu, R. (2002). Molecular linkage maps of the Populus genome. Genome, 45(3), 541-555. https://doi.org/10.1139/g02-013
Yin, Tongming ; Zhang, Xinye ; Huang, Minren ; Wang, Minxiu ; Zhuge, Qiang ; Tu, Shengming ; Zhu, Li Huang ; Wu, Rongling. / Molecular linkage maps of the Populus genome. In: Genome. 2002 ; Vol. 45, No. 3. pp. 541-555.
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abstract = "We report molecular genetic linkage maps for an interspecific hybrid population of Populus, a model system in forest-tree biology. The hybrids were produced by crosses between P. deltoides (mother) and P. euramericana (father), which is a natural hybrid of P. deltoides (grandmother) and P. nigra (grandfather). Linkage analysis from 93 of the 450 backcross progeny grown in the field for 15 years was performed using random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), and inter-simple sequence repeats (ISSRs). Of a total of 839 polymorphic markers identified, 560 (67{\%}) were testcross markers heterozygous in one parent but null in the other (segregating 1:1), 206 (25{\%}) were intercross dominant markers heterozygous in both parents (segregating 3:1), and the remaining 73 (9{\%}) were 19 non-parental RAPD markers (segregating 1:1) and 54 codominant AFLP markers (segregating 1:1:1:1). A mixed set of the testcross markers, non-parental RAPD markers, and codominant AFLP markers was used to construct two linkage maps, one based on the P. deltoides (D) genome and the other based on P. euramericana (E). The two maps showed nearly complete coverage of the genome, spanning 3801 and 3452 cM, respectively. The availability of non-parental RAPD and codominant AFLP markers as orthologous genes allowed for a direct comparison of the rate of meiotic recombination between the two different parental species. Generally, the rate of meiotic recombination was greater for males than females in our interspecific poplar hybrids. The confounded effect of sexes and species causes the mean recombination distance of orthologous markers to be 11{\%} longer for the father (P. euramericana; interspecific hybrid) than for the mother (P. deltoides; pure species). The linkage maps constructed and the interspecific poplar hybrid population in which clonal replicates for individual genotypes are available present a comprehensive foundation for future genomic studies and quantitative trait locus (QTL) identification.",
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Yin, T, Zhang, X, Huang, M, Wang, M, Zhuge, Q, Tu, S, Zhu, LH & Wu, R 2002, 'Molecular linkage maps of the Populus genome', Genome, vol. 45, no. 3, pp. 541-555. https://doi.org/10.1139/g02-013

Molecular linkage maps of the Populus genome. / Yin, Tongming; Zhang, Xinye; Huang, Minren; Wang, Minxiu; Zhuge, Qiang; Tu, Shengming; Zhu, Li Huang; Wu, Rongling.

In: Genome, Vol. 45, No. 3, 08.05.2002, p. 541-555.

Research output: Contribution to journalArticle

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AU - Yin, Tongming

AU - Zhang, Xinye

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AU - Zhuge, Qiang

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AU - Wu, Rongling

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Yin T, Zhang X, Huang M, Wang M, Zhuge Q, Tu S et al. Molecular linkage maps of the Populus genome. Genome. 2002 May 8;45(3):541-555. https://doi.org/10.1139/g02-013