Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster.

R. Whetten, E. Organ, P. Krasney, Diana Lynn Cox-foster, Douglas R. Cavener

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have precisely mapped and sequenced the three 5' exons of the Drosophila melanogaster Gld gene and have identified the start sites for transcription and translation. The first exon is composed of 335 nucleotides and does not contain any putative translation start codons. The second exon is separated from the first exon by 8 kb and contains the Gld translation start codon. The inferred amino acid sequence of the amino terminus contains two unusual features: three tandem repeats of serine-alanine, and a relatively high density of cysteine residues. P element-mediated transformation experiments demonstrated that a 17.5-kb genomic fragment contains the functional and regulatory components of the Gld gene.

Original languageEnglish (US)
Pages (from-to)475-484
Number of pages10
JournalGenetics
Volume120
Issue number2
StatePublished - Jan 1 1988

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Glucose 1-Dehydrogenase
Molecular Structure
Drosophila melanogaster
Exons
Initiator Codon
Genes
Gene Components
Tandem Repeat Sequences
Transcription Initiation Site
Alanine
Serine
Cysteine
Amino Acid Sequence
Nucleotides

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Whetten, R. ; Organ, E. ; Krasney, P. ; Cox-foster, Diana Lynn ; Cavener, Douglas R. / Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster. In: Genetics. 1988 ; Vol. 120, No. 2. pp. 475-484.
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Whetten, R, Organ, E, Krasney, P, Cox-foster, DL & Cavener, DR 1988, 'Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster.', Genetics, vol. 120, no. 2, pp. 475-484.

Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster. / Whetten, R.; Organ, E.; Krasney, P.; Cox-foster, Diana Lynn; Cavener, Douglas R.

In: Genetics, Vol. 120, No. 2, 01.01.1988, p. 475-484.

Research output: Contribution to journalArticle

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