Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

J. R. Petrulis, Gary H. Perdew

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.

Original languageEnglish (US)
Pages (from-to)772-775
Number of pages4
JournalBioTechniques
Volume31
Issue number4
StatePublished - 2001

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Digitonin
Cell Nucleus Active Transport
Green Fluorescent Proteins
Assays
Fusion reactions
Nuclear Localization Signals
Monitoring
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Microscopic examination
Proteins
Visualization
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

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abstract = "A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.",
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Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells. / Petrulis, J. R.; Perdew, Gary H.

In: BioTechniques, Vol. 31, No. 4, 2001, p. 772-775.

Research output: Contribution to journalArticle

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