Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

J. R. Petrulis, Gary H. Perdew

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.

Original languageEnglish (US)
Pages (from-to)772-775
Number of pages4
JournalBioTechniques
Volume31
Issue number4
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

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