Monitoring proteome stress in live cells using halotag-based fluorogenic sensor

Yu Liu, Matthew Fares, Xin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescent folding sensor is a powerful tool to detect proteome stresses, including heat, osmotic, oxidative, and drug induced stresses. Monitoring proteome stress using these sensors allows us to dissect the mechanism of cellular stress and find therapeutics that ameliorate stress related diseases. Here we present a HaloTag-based fluorogenic proteome stress sensor (AgHalo) to robustly detect and quantify proteome stresses in live cells. We describe how proteome stresses are monitored in both bacterial and mammalian live cells using fluorescence confocal microscope and fluorescence plate reader.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages171-182
Number of pages12
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1873
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Liu, Y., Fares, M., & Zhang, X. (2019). Monitoring proteome stress in live cells using halotag-based fluorogenic sensor. In Methods in Molecular Biology (pp. 171-182). (Methods in Molecular Biology; Vol. 1873). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8820-4_10