Monitoring the microbial contamination of beef carcass tissue with a rapid chromogenic Limulus amoebocyte lysate endpoint assay

G. R. Siragusa, D. H. Kang, Catherine Nettles Cutter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A chromogenic Limulus amoebocyte lysate (LAL) endpoint assay was found to be an accurate and rapid means of gauging levels of beef carcass microbial contamination within 10 min. The assay demonstrated a high correlation with the total mesophilic bacterial and coliform surface populations from inoculated beef carcass surface tissues. This assay was tested on a set of actual beef carcass surface samples (n = 121) demonstrating the utility of the chromogenic LAL test as a means of monitoring carcass microbial contamination in a near real-time fashion. Classifying the chromogenic LAL results into four contamination groups was found to be a sound means of utilizing the resultant chromogenic LAL data for detecting carcasses with high levels of microbial contamination. For beef carcass testing, this assay can be used with no instrumentation other than the required 37 °C incubator and, as an option, a microplate reader.

Original languageEnglish (US)
Pages (from-to)178-183
Number of pages6
JournalLetters in Applied Microbiology
Volume31
Issue number2
DOIs
StatePublished - Sep 11 2000

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Endpoint Determination
Horseshoe Crabs
Incubators
Red Meat
endotoxin binding proteins
Population

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cite this

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abstract = "A chromogenic Limulus amoebocyte lysate (LAL) endpoint assay was found to be an accurate and rapid means of gauging levels of beef carcass microbial contamination within 10 min. The assay demonstrated a high correlation with the total mesophilic bacterial and coliform surface populations from inoculated beef carcass surface tissues. This assay was tested on a set of actual beef carcass surface samples (n = 121) demonstrating the utility of the chromogenic LAL test as a means of monitoring carcass microbial contamination in a near real-time fashion. Classifying the chromogenic LAL results into four contamination groups was found to be a sound means of utilizing the resultant chromogenic LAL data for detecting carcasses with high levels of microbial contamination. For beef carcass testing, this assay can be used with no instrumentation other than the required 37 °C incubator and, as an option, a microplate reader.",
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Monitoring the microbial contamination of beef carcass tissue with a rapid chromogenic Limulus amoebocyte lysate endpoint assay. / Siragusa, G. R.; Kang, D. H.; Cutter, Catherine Nettles.

In: Letters in Applied Microbiology, Vol. 31, No. 2, 11.09.2000, p. 178-183.

Research output: Contribution to journalArticle

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