Monoclonal Antibodies to a Glycolipid Antigen on Human Neuroblastoma Cells1

Nai Kong V. Cheung, Ulla M. Saarinen, John Neely, Bonnie Landmeier, Duffy Donovan, Peter F. Coccia

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Abstract

Using a somatic cell hybridization technique, four murine monoclonal antibodies (three immunoglobulin in and one immunoglobulin G3) were produced against a human neuroblastoma cell surface glycolipid antigen. They reacted strongly with all human neuroblastoma tumor-containing specimens and six of eight human neuroblastoma cell lines. More than 98% of each neuroblastoma cell population possessed this surface antigen, and in the presence of complement, 100% of them were killed. While melanoma and osteogenic sarcoma carried this antigen, leukemia and most Ewing's and Wilms' tumors did not. There was no cross-reaction with 30 normal or remission bone marrow samples and none with normal human tissues other than neurons in vitro. This antigen was neuraminidase sensitive, separable on thin-layer chromatogram, and did not modulate after combining with the monoclonal antibodies. These antibodies could detect less than 0.1% tumor cells deliberately seeded in the bone marrow samples. Because of their unique properties, these monoclonal antibodies may have diagnostic and therapeutic potentials.

Original languageEnglish (US)
Pages (from-to)2642-2649
Number of pages8
JournalCancer Research
Volume45
Issue number6
StatePublished - Jun 1 1985

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Cheung, N. K. V., Saarinen, U. M., Neely, J., Landmeier, B., Donovan, D., & Coccia, P. F. (1985). Monoclonal Antibodies to a Glycolipid Antigen on Human Neuroblastoma Cells1. Cancer Research, 45(6), 2642-2649.