Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture

Dana Leifer; Stuart A. Lipton; Colin J. Barnstable; Richard H. Masland

doi:10.1126/science.6143400

Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture

Dana Leifer, Stuart A. Lipton, Colin J. Barnstable, Richard H. Masland

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156 Scopus citations

Abstract

Ganglion cells were dissociated from postnatal rat retinas, identified by specific fluorescent labels, and maintained in culture on a variety of substrates. Regeneration of processes by retinal ganglion cells was enhanced when the cells were plated on glass coated with a monoclonal antibody against the Thy-1 determinant. Plain glass and glass coated with polylysine, collagen, fibronectin, or other monoclonal antibodies supported the growth of neural processes, but were less effective than antibody to Thy-1.

Original language	English (US)
Pages (from-to)	303-306
Number of pages	4
Journal	Science
Volume	224
Issue number	4646
DOIs	https://doi.org/10.1126/science.6143400
Publication status	Published - Jan 1 1984

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Leifer, D., Lipton, S. A., Barnstable, C. J., & Masland, R. H. (1984). Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture. Science, 224(4646), 303-306. https://doi.org/10.1126/science.6143400

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10.1126/science.6143400