Monoclonal antibody VC1.1 selectively stains a population of GABAergic neurons containing the calcium-binding protein parvalbumin in the rat cerebral cortex

T. Kosaka, C. W. Heizmann, Colin Barnstable

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Abstract

Monoclonal antibody VC1.1 is shown to stain selectively a subpopulation of GABAergic neurons in the rat cerebral cortex. Almost all VC1.1 immunoreactive cells were also GABA-like immunoreactive (GABA-LI) and parvalbumin (PV) immunoreactive, whereas they were about 30% and 65% of GABA-LI and PV-positive cells in the parietal cortex and about 13% and 32% in the occipital cortex, respectively. Although a few VC1.1 positive cells showed somatostatinlike and/or cholecystokinin-like immunoreactivities, they were exceptional (less than 1% of VC1.1 positive cells). Furthermore about 90% of VC1.1 positive cells were also stained with a lectin, Vicia villosa agglutinin, with a specific affinity for terminal N-acetylgalactosamine.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalExperimental Brain Research
Volume78
Issue number1
DOIs
StatePublished - Nov 1 1989

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GABAergic Neurons
Parvalbumins
Calcium-Binding Proteins
Cerebral Cortex
Coloring Agents
Monoclonal Antibodies
gamma-Aminobutyric Acid
Population
Acetylgalactosamine
Occipital Lobe
Parietal Lobe
Cholecystokinin
Lectins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Monoclonal antibody VC1.1 is shown to stain selectively a subpopulation of GABAergic neurons in the rat cerebral cortex. Almost all VC1.1 immunoreactive cells were also GABA-like immunoreactive (GABA-LI) and parvalbumin (PV) immunoreactive, whereas they were about 30{\%} and 65{\%} of GABA-LI and PV-positive cells in the parietal cortex and about 13{\%} and 32{\%} in the occipital cortex, respectively. Although a few VC1.1 positive cells showed somatostatinlike and/or cholecystokinin-like immunoreactivities, they were exceptional (less than 1{\%} of VC1.1 positive cells). Furthermore about 90{\%} of VC1.1 positive cells were also stained with a lectin, Vicia villosa agglutinin, with a specific affinity for terminal N-acetylgalactosamine.",
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