Morphology of Puccinia horiana, causal agent of chrysanthemum white rust, sampled from naturally infected plants

G. O’Keefe, Donald Durwood Davis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum × morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur.

Original languageEnglish (US)
Pages (from-to)1738-1743
Number of pages6
JournalPlant disease
Volume99
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Puccinia horiana
Chrysanthemum
teliospores
Chrysanthemum morifolium
basidiospores
stems
appressoria
germ tube
pathotypes
epidermis (plant)
mycelium
leaves
cell walls
cells
flowers
genotype
cultivars
infection

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{782d489b6814409b97ac694825f5a22f,
title = "Morphology of Puccinia horiana, causal agent of chrysanthemum white rust, sampled from naturally infected plants",
abstract = "Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum × morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur.",
author = "G. O’Keefe and Davis, {Donald Durwood}",
year = "2015",
month = "12",
day = "1",
doi = "10.1094/PDIS-02-15-0239-RE",
language = "English (US)",
volume = "99",
pages = "1738--1743",
journal = "Plant Disease",
issn = "0191-2917",
publisher = "American Phytopathological Society",
number = "12",

}

Morphology of Puccinia horiana, causal agent of chrysanthemum white rust, sampled from naturally infected plants. / O’Keefe, G.; Davis, Donald Durwood.

In: Plant disease, Vol. 99, No. 12, 01.12.2015, p. 1738-1743.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Morphology of Puccinia horiana, causal agent of chrysanthemum white rust, sampled from naturally infected plants

AU - O’Keefe, G.

AU - Davis, Donald Durwood

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum × morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur.

AB - Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum × morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur.

UR - http://www.scopus.com/inward/record.url?scp=84951862615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951862615&partnerID=8YFLogxK

U2 - 10.1094/PDIS-02-15-0239-RE

DO - 10.1094/PDIS-02-15-0239-RE

M3 - Article

AN - SCOPUS:84951862615

VL - 99

SP - 1738

EP - 1743

JO - Plant Disease

JF - Plant Disease

SN - 0191-2917

IS - 12

ER -