Morris water maze training in mice elevates hippocampal levels of transcription factors nuclear factor (Erythroid-derived 2)-like 2 and nuclear factor kappa B p65

Wanda M. Snow, Payam S. Pahlavan, Jelena Djordjevic, Danielle McAllister, Eric E. Platt, Shoug Alashmali, Michael J. Bernstein, Miyoung Suh, Benedict C. Albensi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Research has identified several transcription factors that regulate activity-dependent plasticity and memory, with cAMP-response element binding protein (CREB) being the most well-studied. In neurons, CREB activation is influenced by the transcription factor nuclear factor kappa B (NF-κB), considered central to immunity but more recently implicated in memory. The transcription factor early growth response-2 (Egr-2), an NF-κB gene target, is also associated with learning and memory. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an antioxidant transcription factor linked to NF-κB in pathological conditions, has not been studied in normal memory. Given that numerous transcription factors implicated in activity-dependent plasticity demonstrate connections to NF-κB, this study simultaneously evaluated protein levels of NF-κB, CREB, Egr-2, Nrf2, and actin in hippocampi from young (1 month-old) weanling CD1 mice after training in the Morris water maze, a hippocampal-dependent spatial memory task. After a 6-day acquisition period, time to locate the hidden platform decreased in the Morris water maze. Mice spent more time in the target vs. non-target quadrants of the maze, suggestive of recall of the platform location. Western blot data revealed a decrease in NF-κB p50 protein after training relative to controls, whereas NF-κB p65, Nrf2 and actin increased. Nrf2 levels were correlated with platform crosses in nearly all tested animals. These data demonstrate that training in a spatial memory task results in alterations in and associations with particular transcription factors in the hippocampus, including upregulation of NF-κB p65 and Nrf2. Training-induced increases in actin protein levels caution against its use as a loading control in immunoblot studies examining activity-dependent plasticity, learning, and memory.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalFrontiers in Molecular Neuroscience
Volume8
Issue numberNovember
DOIs
StatePublished - Nov 18 2015

Fingerprint

NF-E2 Transcription Factor
NF-kappa B
Water
Transcription Factors
Cyclic AMP Response Element-Binding Protein
Actins
Early Growth Response Transcription Factors
Hippocampus
Learning
Proteins
Immunity
Up-Regulation
Antioxidants
Western Blotting

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Snow, Wanda M. ; Pahlavan, Payam S. ; Djordjevic, Jelena ; McAllister, Danielle ; Platt, Eric E. ; Alashmali, Shoug ; Bernstein, Michael J. ; Suh, Miyoung ; Albensi, Benedict C. / Morris water maze training in mice elevates hippocampal levels of transcription factors nuclear factor (Erythroid-derived 2)-like 2 and nuclear factor kappa B p65. In: Frontiers in Molecular Neuroscience. 2015 ; Vol. 8, No. November. pp. 1-12.
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abstract = "Research has identified several transcription factors that regulate activity-dependent plasticity and memory, with cAMP-response element binding protein (CREB) being the most well-studied. In neurons, CREB activation is influenced by the transcription factor nuclear factor kappa B (NF-κB), considered central to immunity but more recently implicated in memory. The transcription factor early growth response-2 (Egr-2), an NF-κB gene target, is also associated with learning and memory. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an antioxidant transcription factor linked to NF-κB in pathological conditions, has not been studied in normal memory. Given that numerous transcription factors implicated in activity-dependent plasticity demonstrate connections to NF-κB, this study simultaneously evaluated protein levels of NF-κB, CREB, Egr-2, Nrf2, and actin in hippocampi from young (1 month-old) weanling CD1 mice after training in the Morris water maze, a hippocampal-dependent spatial memory task. After a 6-day acquisition period, time to locate the hidden platform decreased in the Morris water maze. Mice spent more time in the target vs. non-target quadrants of the maze, suggestive of recall of the platform location. Western blot data revealed a decrease in NF-κB p50 protein after training relative to controls, whereas NF-κB p65, Nrf2 and actin increased. Nrf2 levels were correlated with platform crosses in nearly all tested animals. These data demonstrate that training in a spatial memory task results in alterations in and associations with particular transcription factors in the hippocampus, including upregulation of NF-κB p65 and Nrf2. Training-induced increases in actin protein levels caution against its use as a loading control in immunoblot studies examining activity-dependent plasticity, learning, and memory.",
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Morris water maze training in mice elevates hippocampal levels of transcription factors nuclear factor (Erythroid-derived 2)-like 2 and nuclear factor kappa B p65. / Snow, Wanda M.; Pahlavan, Payam S.; Djordjevic, Jelena; McAllister, Danielle; Platt, Eric E.; Alashmali, Shoug; Bernstein, Michael J.; Suh, Miyoung; Albensi, Benedict C.

In: Frontiers in Molecular Neuroscience, Vol. 8, No. November, 18.11.2015, p. 1-12.

Research output: Contribution to journalArticle

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T1 - Morris water maze training in mice elevates hippocampal levels of transcription factors nuclear factor (Erythroid-derived 2)-like 2 and nuclear factor kappa B p65

AU - Snow, Wanda M.

AU - Pahlavan, Payam S.

AU - Djordjevic, Jelena

AU - McAllister, Danielle

AU - Platt, Eric E.

AU - Alashmali, Shoug

AU - Bernstein, Michael J.

AU - Suh, Miyoung

AU - Albensi, Benedict C.

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AB - Research has identified several transcription factors that regulate activity-dependent plasticity and memory, with cAMP-response element binding protein (CREB) being the most well-studied. In neurons, CREB activation is influenced by the transcription factor nuclear factor kappa B (NF-κB), considered central to immunity but more recently implicated in memory. The transcription factor early growth response-2 (Egr-2), an NF-κB gene target, is also associated with learning and memory. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an antioxidant transcription factor linked to NF-κB in pathological conditions, has not been studied in normal memory. Given that numerous transcription factors implicated in activity-dependent plasticity demonstrate connections to NF-κB, this study simultaneously evaluated protein levels of NF-κB, CREB, Egr-2, Nrf2, and actin in hippocampi from young (1 month-old) weanling CD1 mice after training in the Morris water maze, a hippocampal-dependent spatial memory task. After a 6-day acquisition period, time to locate the hidden platform decreased in the Morris water maze. Mice spent more time in the target vs. non-target quadrants of the maze, suggestive of recall of the platform location. Western blot data revealed a decrease in NF-κB p50 protein after training relative to controls, whereas NF-κB p65, Nrf2 and actin increased. Nrf2 levels were correlated with platform crosses in nearly all tested animals. These data demonstrate that training in a spatial memory task results in alterations in and associations with particular transcription factors in the hippocampus, including upregulation of NF-κB p65 and Nrf2. Training-induced increases in actin protein levels caution against its use as a loading control in immunoblot studies examining activity-dependent plasticity, learning, and memory.

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