Motility quorum sensing locus (mqsa, B3022) links autoinducer 2 and biofilm formation in Escherichia coli K12

Andres F. González Barrios, Ronjung Zuo, William Bentley, Yoshifumi Hashimoto, Li Yang, Thomas Keith Wood

Research output: Contribution to conferencePaper

Abstract

Previously, the cross-species, quorum-sensing signal AI-2 has been found to induce biofilm formation 30-fold in E. coli K12 (unpublished) by increasing expression of 67 genes, primarily those associated with chemotaxis, motility, and flagellar synthesis (Biotechnol. Bioeng. 88: 630, 2004), including the specific motility loci qseBC and flhD (Mol. Microbiol. 43: 809, 2002). Differential gene expression has also been used to show b3 022 is induced 8-fold in biofilms relative to planktonic cells (Appl. Microbiol. Biotechnol. 64: 515, 2004). In this report we show the b3022 locus, which we have re-named motility quorum sensing (mqsA), regulates motility through the two-component motility regulatory system QseBC. Deletion of mqsA decreased biofilm formation in complex media (74%), minimal media (46%), and complex media supplemented with 0.4% glucose (78%) as well as decreased biomass, substratum coverage, and thickness by 9-, 19-, and 4-fold, respectively, in continuous flow cells. By deleting mqsA, wild-type biofilm architecture was altered from a 54-μm-thick structure with macrocolonies to only a few colonies as visualized by confocal microscopy. MqsA positively regulates expression of qseBC, fliA, and motA since deleting mqsA decreased their transcription by 61-, 2.4-, and 18-fold, respectively, in minimal medium. Biofilm formation was not increased upon the addition of 6.4 μM AI-2 for the isogenic deletions of mqsA, qseBC, fliA, and motA indicating AI-2 induces biofilm formation in E. coli with MqsA as the mediator which regulates QseBC.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

González Barrios, A. F., Zuo, R., Bentley, W., Hashimoto, Y., Yang, L., & Wood, T. K. (2005). Motility quorum sensing locus (mqsa, B3022) links autoinducer 2 and biofilm formation in Escherichia coli K12. Paper presented at 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States.