Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel

Iqbal Ahmad, Christoph Korbmacher, Alan S. Segal, Pamela Cheung, Emile L. Boulpaep, Colin Barnstable

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Apical nonselective cation channels with an average single-channel conductance of 34 ± 2.3 pS were found in M-1 mouse cortical collecting duct cells. Channel activity is increased by depolarization and abolished by cytoplasmic calcium removal. Cytoplasmic application of 0.1 mM cGMP decreases channel open probability by 27%. cDNAs corresponding to ≈40% of the coding region of the photoreceptor channel were isolated by the polymerase chain reaction from M-1 cells and a rat kidney cDNA library. The rat kidneyderived sequence differs by a single base, and the M-1-cell-derived sequence differs by only two bases, from the photoreceptor sequence. A second clone from M-1 cells differs by 20 out of 426 bases from the photoreceptor sequence. In all three clones, the deduced amino acid sequence is identical to that of the rat photoreceptor channel. Northern blot analysis of poly(A)+ RNA from M-1 cells reveals the presence of a 3.2-kilobase band hybridizing with a retinal cGMP-gated cation channel probe. The results suggest the expression in M-1 cells of more than one gene coding for nonselective cation channels or channel subunits, one of which is identical to the cGMP-gated cation channel gene of rod photoreceptors.

Original languageEnglish (US)
Pages (from-to)10262-10266
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
DOIs
StatePublished - Jan 1 1992

Fingerprint

Retinal Rod Photoreceptor Cells
Cations
Genes
Clone Cells
Gene Library
Northern Blotting
Amino Acid Sequence
Complementary DNA
Calcium
Kidney
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{612141469ca4448db4de36b3076b0f5c,
title = "Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel",
abstract = "Apical nonselective cation channels with an average single-channel conductance of 34 ± 2.3 pS were found in M-1 mouse cortical collecting duct cells. Channel activity is increased by depolarization and abolished by cytoplasmic calcium removal. Cytoplasmic application of 0.1 mM cGMP decreases channel open probability by 27{\%}. cDNAs corresponding to ≈40{\%} of the coding region of the photoreceptor channel were isolated by the polymerase chain reaction from M-1 cells and a rat kidney cDNA library. The rat kidneyderived sequence differs by a single base, and the M-1-cell-derived sequence differs by only two bases, from the photoreceptor sequence. A second clone from M-1 cells differs by 20 out of 426 bases from the photoreceptor sequence. In all three clones, the deduced amino acid sequence is identical to that of the rat photoreceptor channel. Northern blot analysis of poly(A)+ RNA from M-1 cells reveals the presence of a 3.2-kilobase band hybridizing with a retinal cGMP-gated cation channel probe. The results suggest the expression in M-1 cells of more than one gene coding for nonselective cation channels or channel subunits, one of which is identical to the cGMP-gated cation channel gene of rod photoreceptors.",
author = "Iqbal Ahmad and Christoph Korbmacher and Segal, {Alan S.} and Pamela Cheung and Boulpaep, {Emile L.} and Colin Barnstable",
year = "1992",
month = "1",
day = "1",
doi = "10.1073/pnas.89.21.10262",
language = "English (US)",
volume = "89",
pages = "10262--10266",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "21",

}

Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel. / Ahmad, Iqbal; Korbmacher, Christoph; Segal, Alan S.; Cheung, Pamela; Boulpaep, Emile L.; Barnstable, Colin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 21, 01.01.1992, p. 10262-10266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel

AU - Ahmad, Iqbal

AU - Korbmacher, Christoph

AU - Segal, Alan S.

AU - Cheung, Pamela

AU - Boulpaep, Emile L.

AU - Barnstable, Colin

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Apical nonselective cation channels with an average single-channel conductance of 34 ± 2.3 pS were found in M-1 mouse cortical collecting duct cells. Channel activity is increased by depolarization and abolished by cytoplasmic calcium removal. Cytoplasmic application of 0.1 mM cGMP decreases channel open probability by 27%. cDNAs corresponding to ≈40% of the coding region of the photoreceptor channel were isolated by the polymerase chain reaction from M-1 cells and a rat kidney cDNA library. The rat kidneyderived sequence differs by a single base, and the M-1-cell-derived sequence differs by only two bases, from the photoreceptor sequence. A second clone from M-1 cells differs by 20 out of 426 bases from the photoreceptor sequence. In all three clones, the deduced amino acid sequence is identical to that of the rat photoreceptor channel. Northern blot analysis of poly(A)+ RNA from M-1 cells reveals the presence of a 3.2-kilobase band hybridizing with a retinal cGMP-gated cation channel probe. The results suggest the expression in M-1 cells of more than one gene coding for nonselective cation channels or channel subunits, one of which is identical to the cGMP-gated cation channel gene of rod photoreceptors.

AB - Apical nonselective cation channels with an average single-channel conductance of 34 ± 2.3 pS were found in M-1 mouse cortical collecting duct cells. Channel activity is increased by depolarization and abolished by cytoplasmic calcium removal. Cytoplasmic application of 0.1 mM cGMP decreases channel open probability by 27%. cDNAs corresponding to ≈40% of the coding region of the photoreceptor channel were isolated by the polymerase chain reaction from M-1 cells and a rat kidney cDNA library. The rat kidneyderived sequence differs by a single base, and the M-1-cell-derived sequence differs by only two bases, from the photoreceptor sequence. A second clone from M-1 cells differs by 20 out of 426 bases from the photoreceptor sequence. In all three clones, the deduced amino acid sequence is identical to that of the rat photoreceptor channel. Northern blot analysis of poly(A)+ RNA from M-1 cells reveals the presence of a 3.2-kilobase band hybridizing with a retinal cGMP-gated cation channel probe. The results suggest the expression in M-1 cells of more than one gene coding for nonselective cation channels or channel subunits, one of which is identical to the cGMP-gated cation channel gene of rod photoreceptors.

UR - http://www.scopus.com/inward/record.url?scp=0026447534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026447534&partnerID=8YFLogxK

U2 - 10.1073/pnas.89.21.10262

DO - 10.1073/pnas.89.21.10262

M3 - Article

C2 - 1279673

AN - SCOPUS:0026447534

VL - 89

SP - 10262

EP - 10266

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 21

ER -