Mouse models for tumor metastasis

Shengyu Yang, J. Jillian Zhang, Xin Yun Huang

Research output: Chapter in Book/Report/Conference proceedingChapter

46 Scopus citations


Tumor metastasis is the main cause of death of cancer patients. Here we describe two mouse models for investigating tumor metastasis. In the first spontaneous metastasis mouse model, 4T1 mouse breast tumor cells are injected into the mammary gland of host mice and the metastasis of 4T1 tumor cells into the lung are examined with a colonogenic assay. In the second experimental metastasis mouse model, luciferase-labeled MDA-MB-231 human breast tumor cells are injected into the tail vein of NOD-SCID immunodeficient mice and the colonization of MDA-MB-231 tumor cells in the lung are monitored using noninvasive bioluminescence imaging.

Original languageEnglish (US)
Title of host publicationRational Drug Design
Subtitle of host publicationMethods and Protocols
EditorsYi Zheng
Number of pages8
StatePublished - Nov 12 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Yang, S., Zhang, J. J., & Huang, X. Y. (2012). Mouse models for tumor metastasis. In Y. Zheng (Ed.), Rational Drug Design: Methods and Protocols (pp. 221-228). (Methods in Molecular Biology; Vol. 928).