Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer

Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy K. Cooper, Sarah A. Brendle, Neil D. Christensen, Todd D. Schell, Jiafen Hu

Research output: Contribution to journalArticle

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Abstract

Mouse papillomavirus has shown broad tissue tropism in nude mice. Previous studies have tested cutaneous infections in different immunocompromised and immunocompetent mouse strains. In the current study, we examined mucosal infection in several immunocompetent and immunocompromised mouse strains. Viral DNA was monitored periodically by Q-PCR of lavage samples. Immunohistochemistry and in situ hybridization were used to determine viral capsid protein and viral DNA respectively. All athymic nude mouse strains showed active infections at both cutaneous and mucosal sites. Interestingly, NOD/SCID mice, which have a deficiency in T, B, and NK cells, showed minimal disease at cutaneous sites but developed persistent infection at the mucosal sites including those of the anogenital region and the oral cavity. Three strains of immunocompetent mice supported mucosal infections. Infections of the lower genital tract in heterozygous (immunocompetent) mice of the NU/J strain progressed to high grade dysplasia and to carcinoma in situ. Anti-MmuPV1 neutralizing antibodies were detected in the sera of all immunocompetent animals. Our findings demonstrate that the mucosae may be the preferred sites for this virus in mice. The mouse model is expected to be a valuable model for the study of mucosal papillomavirus disease, progression, and host immune control.

Original languageEnglish (US)
Article number16932
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Papillomavirus Infections
Mucous Membrane
Neoplasms
Nude Mice
Infection
Viral DNA
Skin
Reproductive Tract Infections
Inbred NOD Mouse
Tropism
SCID Mice
Therapeutic Irrigation
Carcinoma in Situ
Capsid Proteins
Viral Proteins
Neutralizing Antibodies
Natural Killer Cells
In Situ Hybridization
Mouth
Disease Progression

All Science Journal Classification (ASJC) codes

  • General

Cite this

Cladel, Nancy M. ; Budgeon, Lynn R. ; Balogh, Karla K. ; Cooper, Timothy K. ; Brendle, Sarah A. ; Christensen, Neil D. ; Schell, Todd D. ; Hu, Jiafen. / Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer. / Cladel, Nancy M.; Budgeon, Lynn R.; Balogh, Karla K.; Cooper, Timothy K.; Brendle, Sarah A.; Christensen, Neil D.; Schell, Todd D.; Hu, Jiafen.

In: Scientific reports, Vol. 7, No. 1, 16932, 01.12.2017.

Research output: Contribution to journalArticle

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