Mouse papillomavirus infections spread to cutaneous sites with progression to malignancy

Nancy M. Cladel, Lynn R. Budgeon, Timothy K. Cooper, Karla K. Balogh, Neil Christensen, Roland Myers, Vladimir Majerciak, Deanna Gotte, Zhi Ming Zheng, Jiafen Hu

Research output: Contribution to journalArticle

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Abstract

We report secondary cutaneous infections in the mouse papillomavirus (MmuPV1)/mouse model. Our previous study demonstrated that cutaneous MmuPV1 infection could spread to mucosal sites. Recently, we observed that mucosal infections could also spread to various cutaneous sites including the back, tail, muzzle and mammary tissues. The secondary site lesions were positive for viral DNA, viral capsid protein and viral particles as determined by in situ hybridization, immunohistochemistry and transmission electron microscopy analyses, respectively. We also demonstrated differential viral production and tumour growth at different secondarily infected skin sites. For example, fewer viral particles were detected in the least susceptible back tissues when compared with those in the infected muzzle and tail, although similar amounts of viral DNA were detected. Follow-up studies demonstrated that significantly lower amounts of viral DNA were packaged in the back lesions. Lavages harvested from the oral cavity and lower genital tracts were equally infectious at both cutaneous and mucosal sites, supporting the broad tissue tropism of this papillomavirus. Importantly, two secondary skin lesions on the forearms of two mice displayed a malignant phenotype at about 9.5 months post-primary infection. Therefore, MmuPV1 induces not only dysplasia at mucosal sites such as the vagina, anus and oral cavity but also skin carcinoma at cutaneous sites. These findings demonstrate that MmuPV1 mucosal infection can be spread to cutaneous sites and suggest that the model could serve a useful role in the study of the viral life cycle and pathogenesis of papillomavirus.

Original languageEnglish (US)
Article number000926
Pages (from-to)2520-2529
Number of pages10
JournalJournal of General Virology
Volume98
Issue number10
DOIs
StatePublished - Oct 1 2017

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Papillomavirus Infections
Skin
Neoplasms
Viral DNA
Infection
Virion
Mouth
Tail
Tropism
Therapeutic Irrigation
Anal Canal
Capsid Proteins
Viral Proteins
Vagina
Life Cycle Stages
Transmission Electron Microscopy
Coinfection
Forearm
In Situ Hybridization
Breast

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Cladel, Nancy M. ; Budgeon, Lynn R. ; Cooper, Timothy K. ; Balogh, Karla K. ; Christensen, Neil ; Myers, Roland ; Majerciak, Vladimir ; Gotte, Deanna ; Zheng, Zhi Ming ; Hu, Jiafen. / Mouse papillomavirus infections spread to cutaneous sites with progression to malignancy. In: Journal of General Virology. 2017 ; Vol. 98, No. 10. pp. 2520-2529.
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Cladel, NM, Budgeon, LR, Cooper, TK, Balogh, KK, Christensen, N, Myers, R, Majerciak, V, Gotte, D, Zheng, ZM & Hu, J 2017, 'Mouse papillomavirus infections spread to cutaneous sites with progression to malignancy', Journal of General Virology, vol. 98, no. 10, 000926, pp. 2520-2529. https://doi.org/10.1099/jgv.0.000926

Mouse papillomavirus infections spread to cutaneous sites with progression to malignancy. / Cladel, Nancy M.; Budgeon, Lynn R.; Cooper, Timothy K.; Balogh, Karla K.; Christensen, Neil; Myers, Roland; Majerciak, Vladimir; Gotte, Deanna; Zheng, Zhi Ming; Hu, Jiafen.

In: Journal of General Virology, Vol. 98, No. 10, 000926, 01.10.2017, p. 2520-2529.

Research output: Contribution to journalArticle

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