Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis

Gabriela Antúnez De Mayolo, Siela N. Maximova, Sharon Pishak, Mark J. Guiltinan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Previous work in a number of laboratories has led to the development of a highly efficient cacao somatic embryogenesis (SE) protocol, establishing the basis for an Agrobacterium tumefaciens-mediated genetic transformation system. Such a system will be valuable as a basic research tool, and possibly in the future as a means to introduce specific disease resistance or other genes into cacao. However, during our initial attempts at transformation, cacao tissue was frequently destroyed due to Agrobacterium overgrowth following co-cultivation. Furthermore, the addition of the antibiotic cefotaxime, commonly applied to tissue culture media to eliminate Agrobacterium post infection, decreased cacao somatic embryo production by 86%. To define antibiotic conditions sufficient to suppress Agrobacterium while not interfering with cacao SE, we evaluated the efficacy of four antibiotics. Two cephalosporins, cefotaxime and moxalactam; and two penicillins, amoxicillin and carbenicillin. Moxalactam, a β-Lactam antibiotic, was proven to effectively suppress Agrobacterium growth. Furthermore, at certain concentrations, it also significantly enhanced the efficiency of cacao SE. A possible mechanism for this enhancement is proposed.

Original languageEnglish (US)
Pages (from-to)607-615
Number of pages9
JournalPlant Science
Volume164
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

moxalactam
Moxalactam
Agrobacterium
Theobroma cacao
Cacao
somatic embryogenesis
Embryonic Development
antibiotics
Anti-Bacterial Agents
cefotaxime
Cefotaxime
carbenicillin
lactams
cephalosporins
amoxicillin
genetic transformation
penicillins
Agrobacterium radiobacter
somatic embryos
Carbenicillin

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{e936fdce312d4810abef34414c0d78bd,
title = "Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis",
abstract = "Previous work in a number of laboratories has led to the development of a highly efficient cacao somatic embryogenesis (SE) protocol, establishing the basis for an Agrobacterium tumefaciens-mediated genetic transformation system. Such a system will be valuable as a basic research tool, and possibly in the future as a means to introduce specific disease resistance or other genes into cacao. However, during our initial attempts at transformation, cacao tissue was frequently destroyed due to Agrobacterium overgrowth following co-cultivation. Furthermore, the addition of the antibiotic cefotaxime, commonly applied to tissue culture media to eliminate Agrobacterium post infection, decreased cacao somatic embryo production by 86{\%}. To define antibiotic conditions sufficient to suppress Agrobacterium while not interfering with cacao SE, we evaluated the efficacy of four antibiotics. Two cephalosporins, cefotaxime and moxalactam; and two penicillins, amoxicillin and carbenicillin. Moxalactam, a β-Lactam antibiotic, was proven to effectively suppress Agrobacterium growth. Furthermore, at certain concentrations, it also significantly enhanced the efficiency of cacao SE. A possible mechanism for this enhancement is proposed.",
author = "{Ant{\'u}nez De Mayolo}, Gabriela and Maximova, {Siela N.} and Sharon Pishak and Guiltinan, {Mark J.}",
year = "2003",
month = "4",
day = "1",
doi = "10.1016/S0168-9452(03)00012-8",
language = "English (US)",
volume = "164",
pages = "607--615",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "4",

}

Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis. / Antúnez De Mayolo, Gabriela; Maximova, Siela N.; Pishak, Sharon; Guiltinan, Mark J.

In: Plant Science, Vol. 164, No. 4, 01.04.2003, p. 607-615.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis

AU - Antúnez De Mayolo, Gabriela

AU - Maximova, Siela N.

AU - Pishak, Sharon

AU - Guiltinan, Mark J.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Previous work in a number of laboratories has led to the development of a highly efficient cacao somatic embryogenesis (SE) protocol, establishing the basis for an Agrobacterium tumefaciens-mediated genetic transformation system. Such a system will be valuable as a basic research tool, and possibly in the future as a means to introduce specific disease resistance or other genes into cacao. However, during our initial attempts at transformation, cacao tissue was frequently destroyed due to Agrobacterium overgrowth following co-cultivation. Furthermore, the addition of the antibiotic cefotaxime, commonly applied to tissue culture media to eliminate Agrobacterium post infection, decreased cacao somatic embryo production by 86%. To define antibiotic conditions sufficient to suppress Agrobacterium while not interfering with cacao SE, we evaluated the efficacy of four antibiotics. Two cephalosporins, cefotaxime and moxalactam; and two penicillins, amoxicillin and carbenicillin. Moxalactam, a β-Lactam antibiotic, was proven to effectively suppress Agrobacterium growth. Furthermore, at certain concentrations, it also significantly enhanced the efficiency of cacao SE. A possible mechanism for this enhancement is proposed.

AB - Previous work in a number of laboratories has led to the development of a highly efficient cacao somatic embryogenesis (SE) protocol, establishing the basis for an Agrobacterium tumefaciens-mediated genetic transformation system. Such a system will be valuable as a basic research tool, and possibly in the future as a means to introduce specific disease resistance or other genes into cacao. However, during our initial attempts at transformation, cacao tissue was frequently destroyed due to Agrobacterium overgrowth following co-cultivation. Furthermore, the addition of the antibiotic cefotaxime, commonly applied to tissue culture media to eliminate Agrobacterium post infection, decreased cacao somatic embryo production by 86%. To define antibiotic conditions sufficient to suppress Agrobacterium while not interfering with cacao SE, we evaluated the efficacy of four antibiotics. Two cephalosporins, cefotaxime and moxalactam; and two penicillins, amoxicillin and carbenicillin. Moxalactam, a β-Lactam antibiotic, was proven to effectively suppress Agrobacterium growth. Furthermore, at certain concentrations, it also significantly enhanced the efficiency of cacao SE. A possible mechanism for this enhancement is proposed.

UR - http://www.scopus.com/inward/record.url?scp=0038442850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038442850&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(03)00012-8

DO - 10.1016/S0168-9452(03)00012-8

M3 - Article

AN - SCOPUS:0038442850

VL - 164

SP - 607

EP - 615

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 4

ER -